Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | activity is dependent on a redox-active cysteine at the active centre | Trypanosoma cruzi |
Cloned (Comment) | Organism |
---|---|
overexpression of wild-type and mutants C53S, C53A, C58S in Escherichia coli | Trypanosoma cruzi |
Protein Variants | Comment | Organism |
---|---|---|
C53A | site-directed mutagenesis, without catalytic active cysteine residue in the active center, no activity with trypanothione disulfide and NADPH, but showing transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi |
C53S | site-directed mutagenesis, without catalytic active cysteine residue in the active center, no activity with trypanothione disulfide and NADPH, but showing transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi |
C58S | site-directed mutagenesis, without catalytic active cysteine residue in the active center, no activity with trypanothione disulfide and NADPH, but showing transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | 0.015 | thio-NADP+ | recombinant mutants C53S, C53A, C58S | Trypanosoma cruzi | |
0.007 | 0.014 | NADPH | recombinant mutants C53S, C53A, C58S | Trypanosoma cruzi | |
0.017 | - |
NADPH | recombinant wild-type | Trypanosoma cruzi | |
0.024 | - |
trypanothione disulfide | recombinant wild-type | Trypanosoma cruzi | |
0.046 | - |
glutathionylspermidine disulfide | recombinant wild-type | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
Silvio strain | - |
Trypanosoma cruzi Silvio | - |
Silvio strain | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutants C53S, C53A, C58S from Escherichia coli | Trypanosoma cruzi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.18 | - |
recombinant mutant C58S: transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi |
0.21 | - |
recombinant mutant C53A: transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi |
0.44 | - |
recombinant mutant C53S: transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi |
6.49 | - |
recombinant wild-type: transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi |
143.2 | - |
purified recombinant enzyme | Trypanosoma cruzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathionylspermidine disulfide + NADPH | - |
Trypanosoma cruzi | 2-glutathionylspermidine + NADP+ | - |
? | |
glutathionylspermidine disulfide + NADPH | - |
Trypanosoma cruzi Silvio | 2-glutathionylspermidine + NADP+ | - |
? | |
additional information | wild-type enzyme shows transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi | ? | - |
? | |
additional information | recombinant mutants C53S, C53A, C58S: no activity with trypanothione disulfide and NADPH, but showing transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi | ? | - |
? | |
additional information | wild-type enzyme shows transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi Silvio | ? | - |
? | |
additional information | recombinant mutants C53S, C53A, C58S: no activity with trypanothione disulfide and NADPH, but showing transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi Silvio | ? | - |
? | |
NADPH + H+ + thio-NADP+ | - |
Trypanosoma cruzi | NADP+ + thio-NADPH + H+ | - |
r | |
NADPH + H+ + thio-NADP+ | - |
Trypanosoma cruzi Silvio | NADP+ + thio-NADPH + H+ | - |
r | |
trypanothione disulfide + NADPH | - |
Trypanosoma cruzi | trypanothione + NADP+ | - |
? | |
trypanothione disulfide + NADPH | - |
Trypanosoma cruzi Silvio | trypanothione + NADP+ | - |
? | |
trypanothione disulfide + thio-NADPH + H+ | - |
Trypanosoma cruzi | trypanothione + thio-NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
TR | - |
Trypanosoma cruzi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Trypanosoma cruzi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | 0.47 | thio-NADPH | recombinant mutants C53S, C53A, C58S | Trypanosoma cruzi | |
0.21 | 0.51 | NADPH | recombinant mutants C53S, C53A, C58S | Trypanosoma cruzi | |
6.33 | - |
NADPH | recombinant wild-type | Trypanosoma cruzi | |
7.02 | - |
thio-NADPH | recombinant wild-type | Trypanosoma cruzi | |
85.4 | - |
glutathionylspermidine disulfide | recombinant wild-type | Trypanosoma cruzi | |
108 | - |
trypanothione disulfide | recombinant wild-type | Trypanosoma cruzi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
beta-thio-NADPH | - |
Trypanosoma cruzi | |
FAD | - |
Trypanosoma cruzi | |
NADPH | - |
Trypanosoma cruzi |