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Literature summary for 1.8.1.11 extracted from
- Asparagusate dehydrogenases and lipoyl dehydrogenase from Asparagus mitochondria (1975), Biochim. Biophys. Acta, 384, 342-352.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | reduction of lipoic acid is activated | Asparagus officinalis |  |
| sodium dodecylsulfate | treatment of mitochondria with at 0.5% for 10-20 min, maximal solubilization of enzyme | Asparagus officinalis | |
General Stability
| General Stability | Organism |
|---|---|
| EDTA ion stabilizes the enzyme | Asparagus officinalis |
| PO43- stabilizes the enzyme | Asparagus officinalis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| asparagusic acid | inhibits lipoyl dehydrogenase activity | Asparagus officinalis | |
| NAD+ | inactivator during asparagusic acid reduction | Asparagus officinalis | |
| NADH | - |
Asparagus officinalis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Asparagus officinalis | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| asparagusate + NADH | Asparagus officinalis | participates in enzymatic pyruvate and alpha-ketoglutarate dehydrogenation | dihydroasparagusate + NAD+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Asparagus officinalis | - |
2 asparagusate dehydrogenases with lipoyl dehydrogenase activity | - |
| Asparagus officinalis | - |
etiolated | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| SDS-extraction, gel filtration, ion-exchange | Asparagus officinalis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| shoot | - |
Asparagus officinalis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.064 | - |
enzyme/fraction I towards asparagusic acid | Asparagus officinalis |
| 0.08323 | - |
enzyme/fraction II towards asparagusic acid | Asparagus officinalis |
| 0.1631 | - |
enzyme/fraction I towards lipoic acid | Asparagus officinalis |
| 0.213 | - |
enzyme/fraction II towards lipoic acid | Asparagus officinalis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, 67 mM sodium phosphate, pH 7.0, no loss of activity for at least 1 month | Asparagus officinalis |
| 4°C, 67 mM sodium phosphate, pH 7.0, very unstable, complete loss of activity in two weeks | Asparagus officinalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| asparagusate + NADH | participates in enzymatic pyruvate and alpha-ketoglutarate dehydrogenation | Asparagus officinalis | dihydroasparagusate + NAD+ | - |
r | |
| asparagusic acid + NADH | - |
Asparagus officinalis | dihydroasparagusate + NAD+ | - |
r | |
| lipoic acid + NADH | - |
Asparagus officinalis | dihydrolipoic acid + NAD+ | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Asparagus officinalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.95 | - |
assay at | Asparagus officinalis |
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