Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NAD+ | reduction of lipoic acid is activated | Asparagus officinalis | |
sodium dodecylsulfate | treatment of mitochondria with at 0.5% for 10-20 min, maximal solubilization of enzyme | Asparagus officinalis |
General Stability | Organism |
---|---|
EDTA ion stabilizes the enzyme | Asparagus officinalis |
PO43- stabilizes the enzyme | Asparagus officinalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
asparagusic acid | inhibits lipoyl dehydrogenase activity | Asparagus officinalis | |
NAD+ | inactivator during asparagusic acid reduction | Asparagus officinalis | |
NADH | - |
Asparagus officinalis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Asparagus officinalis | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
asparagusate + NADH | Asparagus officinalis | participates in enzymatic pyruvate and alpha-ketoglutarate dehydrogenation | dihydroasparagusate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Asparagus officinalis | - |
2 asparagusate dehydrogenases with lipoyl dehydrogenase activity | - |
Asparagus officinalis | - |
etiolated | - |
Purification (Comment) | Organism |
---|---|
SDS-extraction, gel filtration, ion-exchange | Asparagus officinalis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
shoot | - |
Asparagus officinalis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.064 | - |
enzyme/fraction I towards asparagusic acid | Asparagus officinalis |
0.08323 | - |
enzyme/fraction II towards asparagusic acid | Asparagus officinalis |
0.1631 | - |
enzyme/fraction I towards lipoic acid | Asparagus officinalis |
0.213 | - |
enzyme/fraction II towards lipoic acid | Asparagus officinalis |
Storage Stability | Organism |
---|---|
-80°C, 67 mM sodium phosphate, pH 7.0, no loss of activity for at least 1 month | Asparagus officinalis |
4°C, 67 mM sodium phosphate, pH 7.0, very unstable, complete loss of activity in two weeks | Asparagus officinalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
asparagusate + NADH | participates in enzymatic pyruvate and alpha-ketoglutarate dehydrogenation | Asparagus officinalis | dihydroasparagusate + NAD+ | - |
r | |
asparagusic acid + NADH | - |
Asparagus officinalis | dihydroasparagusate + NAD+ | - |
r | |
lipoic acid + NADH | - |
Asparagus officinalis | dihydrolipoic acid + NAD+ | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Asparagus officinalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.95 | - |
assay at | Asparagus officinalis |