Application | Comment | Organism |
---|---|---|
medicine | potential point of intervention in the treatment of staphylococcal infections | Staphylococcus aureus |
pharmacology | anti-staphylococcal agent, possible target for the design of selective inhibitors that would interrupt the thiol metabolism of the human pathogen Staphylococcus aureus | Staphylococcus aureus |
Cloned (Comment) | Organism |
---|---|
cdr gene encoding coenzyme A glutathione disulfide reductase is cloned, sequenced and overexpressed in Escherichia coli BL21 using the pET expression system, gene encodes a 438-amino acid polypeptide | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
NADPH | recombinant enzyme expressed in E. coli | Staphylococcus aureus | |
0.014 | - |
CoA disulfide | recombinant enzyme expressed in E. coli | Staphylococcus aureus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49200 | - |
2 * 49200, calculated from the nucleotide sequence | Staphylococcus aureus |
90000 | - |
native molecular weight | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA disulfide + NADPH | Staphylococcus aureus | central role in thiol metabolism | CoA + NADP+ | - |
? | |
CoA disulfide + NADPH | Staphylococcus aureus NCTC 8325 | central role in thiol metabolism | CoA + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
strain 8325-4 | - |
Staphylococcus aureus NCTC 8325 | - |
strain 8325-4 | - |
Purification (Comment) | Organism |
---|---|
purification of recombinant CoADR, expressed in Escherichia coli | Staphylococcus aureus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA disulfide + NADPH | substrate CoASSCoA, enzyme forms during catalysis a stable mixed disulfide intermediate with CoA | Staphylococcus aureus | CoA + NADP+ | - |
? | |
CoA disulfide + NADPH | central role in thiol metabolism | Staphylococcus aureus | CoA + NADP+ | - |
? | |
CoA disulfide + NADPH | substrate CoASSCoA, enzyme forms during catalysis a stable mixed disulfide intermediate with CoA | Staphylococcus aureus NCTC 8325 | CoA + NADP+ | - |
? | |
CoA disulfide + NADPH | central role in thiol metabolism | Staphylococcus aureus NCTC 8325 | CoA + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 49200, calculated from the nucleotide sequence | Staphylococcus aureus |
Synonyms | Comment | Organism |
---|---|---|
CoA disulfide reductase | - |
Staphylococcus aureus |
CoADR | - |
Staphylococcus aureus |
More | subfamily of pyridine nucleotide-disulfide oxidoreductase | Staphylococcus aureus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1100 | - |
CoA disulfide | - |
Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein, gene with two FAD-binding regions | Staphylococcus aureus | |
NADPH | NADPH-dependent | Staphylococcus aureus | |
NADPH | gene with one NADPH-binding region | Staphylococcus aureus |