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Literature summary for 1.7.99.1 extracted from

  • Hucklesby, D.P.; Hewitt, E.J.
    Nitrite and hydroxylamine reduction in higher plants. Fractionation, electron donor and substrate specificity of leaf enzymes, principally from vegetable marrow (Cucurbita pepo L.) (1970), Biochem. J., 119, 615-627.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.7.99.1

Inhibitors

Inhibitors Comment Organism Structure
Guanidinium chloride 25% inhibition at 2 mM, 30% inhibition at 3 mM Cucurbita pepo

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 hydroxylamine reductase 2, gel filtration Cucurbita pepo
60000
-
 hydroxylamine reductase 1, gel filtration Cucurbita pepo

Organism

Organism UniProt Comment Textmining
Cucurbita pepo
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cucurbita pepo

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Cucurbita pepo
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 specific activities after different purification methods Cucurbita pepo

Storage Stability

Storage Stability Organism
-18°C, DEAE-cellulose fraction, often stable for several weeks, but unpredictable loss of activity Cucurbita pepo

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hydroxylamine + reduced acceptor hydroxylamine reductase 1 reacts with reduced benzyl viologen, ferredoxin and FMNH2 Cucurbita pepo ammonia + oxidized acceptor
-
 ?
hydroxylamine + reduced acceptor hydroxylamine reductase 2 reacts with benzyl viologen and dithionite Cucurbita pepo ammonia + oxidized acceptor
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 10 min, 25-40% loss of activity Cucurbita pepo
60
-
 10 min, 50-85% loss of activity Cucurbita pepo

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 no clear or consistent pH optima observed with hydroxylamine reductase 1 and hydroxylamine reductase 2 Cucurbita pepo