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Literature summary for 1.7.7.2 extracted from

  • Srivastava, A.P.; Mishra, N.; Prasad, R.L.A.; Rajesh, P.; Knaff, D.B.
    Thermodynamics of ferredoxin binding to cyanobacterial nitrate reductase (2020), Photosynth. Res., 144, 73-84 .
    View publication on PubMed
Search for more literature for 1.7.7.2

Protein Variants

Protein Variants Comment Organism
D60E the mutant shows 71.3% activity compared to the wild type enzyme Synechocystis sp. PCC 6803
D60N the mutant shows 41.5% activity compared to the wild type enzyme Synechocystis sp. PCC 6803
D65N the mutant shows 97% activity compared to the wild type enzyme Synechocystis sp. PCC 6803
D66N the mutant shows 92% activity compared to the wild type enzyme Synechocystis sp. PCC 6803
E29D the mutant shows 84.8% activity compared to the wild type enzyme Synechocystis sp. PCC 6803
E29N the mutant shows 59% activity compared to the wild type enzyme Synechocystis sp. PCC 6803
E30N the mutant shows 92.7% activity compared to the wild type enzyme Synechocystis sp. PCC 6803
E92D the mutant shows 40.5% activity compared to the wild type enzyme Synechocystis sp. PCC 6803
E92Q the mutant shows less than 0.1% activity compared to the wild type enzyme Synechocystis sp. PCC 6803
E93D the mutant shows 113% activity compared to the wild type enzyme Synechocystis sp. PCC 6803
E93N the mutant shows 46% activity compared to the wild type enzyme Synechocystis sp. PCC 6803

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.038
-
 reduced ferredoxin wild type enzyme, at pH 8.0 and 25°C Synechocystis sp. PCC 6803
0.047
-
 reduced ferredoxin mutant enzyme E93D, at pH 8.0 and 25°C Synechocystis sp. PCC 6803
0.052
-
 reduced ferredoxin mutant enzyme D66N, at pH 8.0 and 25°C Synechocystis sp. PCC 6803
0.056
-
 reduced ferredoxin mutant enzyme D65N, at pH 8.0 and 25°C Synechocystis sp. PCC 6803
0.057
-
 reduced ferredoxin mutant enzyme E30N, at pH 8.0 and 25°C Synechocystis sp. PCC 6803
0.059
-
 reduced ferredoxin mutant enzyme E29D, at pH 8.0 and 25°C Synechocystis sp. PCC 6803
0.065
-
 reduced ferredoxin mutant enzyme E29N, at pH 8.0 and 25°C Synechocystis sp. PCC 6803
0.076
-
 reduced ferredoxin mutant enzyme D60E, at pH 8.0 and 25°C Synechocystis sp. PCC 6803
0.077
-
 reduced ferredoxin mutant enzyme E93N, at pH 8.0 and 25°C Synechocystis sp. PCC 6803
0.089
-
 reduced ferredoxin mutant enzyme D60N, at pH 8.0 and 25°C Synechocystis sp. PCC 6803
0.132
-
 reduced ferredoxin mutant enzyme E92D, at pH 8.0 and 25°C Synechocystis sp. PCC 6803

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrate + 2 reduced ferredoxin + 2 H+ Synechocystis sp. PCC 6803
-
 nitrite + H2O + 2 oxidized ferredoxin
-
 ?

Organism

Organism UniProt Comment Textmining
Synechocystis sp. PCC 6803
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrate + 2 reduced ferredoxin + 2 H+
-
 Synechocystis sp. PCC 6803 nitrite + H2O + 2 oxidized ferredoxin
-
 ?