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Literature summary for 1.7.7.2 extracted from

  • Kameya, M.; Kanbe, H.; Igarashi, Y.; Arai, H.; Ishii, M.
    Nitrate reductases in Hydrogenobacter thermophilus with evolutionarily ancient features distinctive localization and electron transfer (2017), Mol. Microbiol., 106, 129-141 .
    View publication on PubMed
Search for more literature for 1.7.7.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Hydrogenobacter thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 reduced ferredoxin 1 at pH 8.0 and 70°C Hydrogenobacter thermophilus

Organism

Organism UniProt Comment Textmining
Hydrogenobacter thermophilus
-
-
-
Hydrogenobacter thermophilus TK-6
-
-
-

Purification (Commentary)

Purification (Comment) Organism
butyl-Toyopearl column chromatography, DEAE-Toyopearl column chromatography, hydroxyapatite column chromatography and MonoQ column chromatography Hydrogenobacter thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.019
-
 crude extract, with ferredoxin 1 as electron donor, at pH 8.0 and 70°C Hydrogenobacter thermophilus
14
-
 after 758fold purification, with ferredoxin 1 as electron donor, at pH 8.0 and 70°C Hydrogenobacter thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no significant nitrate-reducing activity is detected when NADH or NADPH is used as the electron donor. The enzyme also shows no significant reactivity toward methylviologen, benzyl viologen or ferredoxin 3 Hydrogenobacter thermophilus ?
-
-
additional information no significant nitrate-reducing activity is detected when NADH or NADPH is used as the electron donor. The enzyme also shows no significant reactivity toward methylviologen, benzyl viologen or ferredoxin 3 Hydrogenobacter thermophilus TK-6 ?
-
-
nitrate + 2 reduced ferredoxin 1 + 2 H+
-
 Hydrogenobacter thermophilus nitrite + H2O + 2 oxidized ferredoxin 1
-
 ?
nitrate + 2 reduced ferredoxin 1 + 2 H+
-
 Hydrogenobacter thermophilus TK-6 nitrite + H2O + 2 oxidized ferredoxin 1
-
 ?
nitrate + 2 reduced ferredoxin 2 + 2 H+
-
 Hydrogenobacter thermophilus nitrite + H2O + 2 oxidized ferredoxin 2
-
 ?
nitrate + 2 reduced ferredoxin 2 + 2 H+
-
 Hydrogenobacter thermophilus TK-6 nitrite + H2O + 2 oxidized ferredoxin 2
-
 ?

Subunits

Subunits Comment Organism
? x * 77000, SDS-PAGE Hydrogenobacter thermophilus

Synonyms

Synonyms Comment Organism
Assimilatory nitrate reductase
-
 Hydrogenobacter thermophilus
Fd-NAS
-
 Hydrogenobacter thermophilus
NAS
-
 Hydrogenobacter thermophilus
NasB
-
 Hydrogenobacter thermophilus

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.4 the enzyme has high activity between pH 7.5 and 8.4 Hydrogenobacter thermophilus

General Information

General Information Comment Organism
metabolism the enzyme is necessary for nitrate assimilation Hydrogenobacter thermophilus