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Literature summary for 1.7.7.2 extracted from
- The assimilatory nitrate-reducing system and its regulation (1981), Annu. Rev. Plant Physiol., 32, 169-204.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Cyanate | stimulates | Acinetobacter calcoaceticus |  |
| Cyanate | stimulates | Azotobacter chroococcum | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cyanide | - |
Acinetobacter calcoaceticus | |
| cyanide | - |
Azotobacter chroococcum | |
| cyanide | powerful inhibitor | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| p-hydroxymercuribenzoate | - |
Acinetobacter calcoaceticus | |
| p-hydroxymercuribenzoate | - |
Azotobacter chroococcum | |
| p-hydroxymercuribenzoate | powerful inhibitor | Synechococcus elongatus PCC 7942 = FACHB-805 | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
reduced ferredoxin | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 0.69 | - |
nitrate | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | tightly bound to chlorophyll-containing membrane fractions | Synechococcus elongatus PCC 7942 = FACHB-805 | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Molybdenum | molybdoprotein | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| Molybdenum | molybdoprotein | Acinetobacter calcoaceticus | |
| Molybdenum | molybdoprotein | Rhodobacter capsulatus | |
| Molybdenum | molybdoprotein | Azotobacter chroococcum | |
| Molybdenum | enzyme contains one molybdenum atom | Rhodobacter capsulatus | |
| Molybdenum | essential role in catalytic activity | Synechococcus elongatus PCC 7942 = FACHB-805 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
- |
Synechococcus elongatus PCC 7942 = FACHB-805 |
| 85000 | - |
2 * 85000 | Rhodobacter capsulatus |
| 100000 | - |
about | Acinetobacter calcoaceticus |
| 100000 | - |
about | Azotobacter chroococcum |
| 185000 | - |
- |
Rhodobacter capsulatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrate + reduced ferredoxin | Synechococcus elongatus PCC 7942 = FACHB-805 | assimilatory nitrate reduction | nitrite + oxidized ferredoxin | - |
? | |
| nitrate + reduced ferredoxin | Anabaena cylindrica | assimilatory nitrate reduction | nitrite + oxidized ferredoxin | - |
? | |
| nitrate + reduced ferredoxin | Desmonostoc muscorum | assimilatory nitrate reduction | nitrite + oxidized ferredoxin | - |
? | |
| nitrate + reduced ferredoxin | Synechococcus elongatus PCC 7942 = FACHB-805 | reduced ferredoxin as physiological electron donor | nitrite + oxidized ferredoxin | - |
? | |
| nitrate + reduced ferredoxin | Anabaena cylindrica | reduced ferredoxin as physiological electron donor | nitrite + oxidized ferredoxin | - |
? | |
| nitrate + reduced ferredoxin | Desmonostoc muscorum | reduced ferredoxin as physiological electron donor | nitrite + oxidized ferredoxin | - |
? | |
| nitrate + reduced flavodoxin | Synechococcus elongatus PCC 7942 = FACHB-805 | flavodoxin can physiologically substitute for ferredoxin under conditions of iron starvation | nitrite + oxidized flavodoxin + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter calcoaceticus | - |
- |
- |
| Anabaena cylindrica | - |
- |
- |
| Azotobacter chroococcum | - |
- |
- |
| Desmonostoc muscorum | - |
- |
- |
| Rhodobacter capsulatus | - |
- |
- |
| Synechococcus elongatus PCC 7942 = FACHB-805 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Synechococcus elongatus PCC 7942 = FACHB-805 |
- |
Rhodobacter capsulatus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Synechococcus elongatus PCC 7942 = FACHB-805 |
| additional information | - |
- |
Rhodobacter capsulatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrate + reduced ferredoxin | - |
Acinetobacter calcoaceticus | nitrite + H2O + oxidized ferredoxin | - |
? | |
| nitrate + reduced ferredoxin | - |
Rhodobacter capsulatus | nitrite + H2O + oxidized ferredoxin | - |
? | |
| nitrate + reduced ferredoxin | - |
Azotobacter chroococcum | nitrite + H2O + oxidized ferredoxin | - |
? | |
| nitrate + reduced ferredoxin | enzyme can not accept electrons directly from NAD(P)H, it is dependent on reduced ferredoxin as physiological electron donor | Synechococcus elongatus PCC 7942 = FACHB-805 | nitrite + H2O + oxidized ferredoxin | - |
? | |
| nitrate + reduced ferredoxin | enzyme can not accept electrons directly from NAD(P)H, it is dependent on reduced ferredoxin as physiological electron donor | Anabaena cylindrica | nitrite + H2O + oxidized ferredoxin | - |
? | |
| nitrate + reduced ferredoxin | enzyme can not accept electrons directly from NAD(P)H, it is dependent on reduced ferredoxin as physiological electron donor | Desmonostoc muscorum | nitrite + H2O + oxidized ferredoxin | - |
? | |
| nitrate + reduced ferredoxin | assimilatory nitrate reduction | Synechococcus elongatus PCC 7942 = FACHB-805 | nitrite + oxidized ferredoxin | - |
? | |
| nitrate + reduced ferredoxin | assimilatory nitrate reduction | Anabaena cylindrica | nitrite + oxidized ferredoxin | - |
? | |
| nitrate + reduced ferredoxin | assimilatory nitrate reduction | Desmonostoc muscorum | nitrite + oxidized ferredoxin | - |
? | |
| nitrate + reduced ferredoxin | reduced ferredoxin as physiological electron donor | Synechococcus elongatus PCC 7942 = FACHB-805 | nitrite + oxidized ferredoxin | - |
? | |
| nitrate + reduced ferredoxin | reduced ferredoxin as physiological electron donor | Anabaena cylindrica | nitrite + oxidized ferredoxin | - |
? | |
| nitrate + reduced ferredoxin | reduced ferredoxin as physiological electron donor | Desmonostoc muscorum | nitrite + oxidized ferredoxin | - |
? | |
| nitrate + reduced flavodoxin | flavodoxin can physiologically substitute for ferredoxin under conditions of iron starvation | Synechococcus elongatus PCC 7942 = FACHB-805 | nitrite + oxidized flavodoxin + H2O | - |
? | |
| nitrate + reduced methyl viologen | enzyme uses also reduced methyl viologen as reductant | Synechococcus elongatus PCC 7942 = FACHB-805 | nitrite + oxidized methyl viologen | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 85000 | Rhodobacter capsulatus |
| monomer | 1 * 75000 | Synechococcus elongatus PCC 7942 = FACHB-805 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1090 | - |
nitrate | dithionite-reduced methyl viologen as electron donor | Synechococcus elongatus PCC 7942 = FACHB-805 | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10.5 | - |
dithionite-reduced methyl viologen as electron donor | Synechococcus elongatus PCC 7942 = FACHB-805 |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme c | enzyme contains 1.5 heme molecules of the c-type | Rhodobacter capsulatus | |
| additional information | no presence of flavin, heme or cytochrome | Synechococcus elongatus PCC 7942 = FACHB-805 |
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