Cloned (Comment) | Organism |
---|---|
- |
Nicotiana tabacum |
expression in Escherichia coli | Nicotiana tabacum |
Crystallization (Comment) | Organism |
---|---|
wild-type, to 1.25 A resolution and mutants Q448K, M175E, M175G, M175K to 2.0, 1.7. 1.7, 19 A resolution, respectively. The structure provides detailed geometries for the [4Fe4S] cluster and the siroheme prosthetic groups | Nicotiana tabacum |
Protein Variants | Comment | Organism |
---|---|---|
K449Q | mutation does not affect enzymatic activity | Nicotiana tabacum |
M175E | the conformation of the Gln47 and Lys91 side-chains changes significantly. Compared with the conformation in wild-type, the Gln47 side-chain rotates about 120 degrees and the amino group in the Lys91 side-chain moves toward the side-chain of residue 175 | Nicotiana tabacum |
M175G | the conformation of the Gln47 and Lys91 side-chains changes significantly. Compared with the conformation in wild-type, the Gln47 side-chain rotates about 120 degrees and the amino group in the Lys91 side-chain moves toward the side-chain of residue 175 | Nicotiana tabacum |
M175K | the conformation of the Gln47 and Lys91 side-chains changes significantly. Compared with the conformation in wild-type, the Gln47 side-chain rotates about 120 degrees and the amino group in the Lys91 side-chain moves toward the side-chain of residue 175 | Nicotiana tabacum |
Q448K | mutation had only a negligible effect on the overall fold of the protein. In addition, the mutation did not affect the hydrogen bond interaction at the distal position of the siroheme | Nicotiana tabacum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.156 | - |
nitrite | wild-type, pH 7.0, 30°C | Nicotiana tabacum | |
0.226 | - |
nitrite | mutant Q448K, pH 7.0, 30°C | Nicotiana tabacum | |
0.277 | - |
nitrite | mutant K449Q, pH 7.0, 30°C | Nicotiana tabacum | |
0.587 | - |
nitrite | wild-type, pH 7.0, 30°C | Nicotiana tabacum | |
0.76 | - |
nitrite | mutant M175GK pH 7.0, 30°C | Nicotiana tabacum | |
0.767 | - |
nitrite | mutant M175E, pH 7.0, 30°C | Nicotiana tabacum | |
1.087 | - |
nitrite | mutant M175G, pH 7.0, 30°C | Nicotiana tabacum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63700 | - |
x * 63700, calculated | Nicotiana tabacum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | Q76KA9 | isoform nii4 | - |
Nicotiana tabacum | Q76KB0 | isoform nii3 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Nicotiana tabacum | - |
root | - |
Nicotiana tabacum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrite + reduced methyl viologen | - |
Nicotiana tabacum | NH3 + H2O + oxidized methyl viologen | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 63700, calculated | Nicotiana tabacum |
Synonyms | Comment | Organism |
---|---|---|
aNiR | - |
Nicotiana tabacum |
assimilatory nitrite reductase | - |
Nicotiana tabacum |
nii3 | - |
Nicotiana tabacum |
nii4 | - |
Nicotiana tabacum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
73 | - |
nitrite | wild-type, pH 7.0, 30°C | Nicotiana tabacum | |
92 | - |
nitrite | mutant K449Q, pH 7.0, 30°C | Nicotiana tabacum | |
96 | - |
nitrite | mutant M175E, pH 7.0, 30°C | Nicotiana tabacum | |
102 | - |
nitrite | mutant M175GK pH 7.0, 30°C | Nicotiana tabacum | |
107 | - |
nitrite | mutant Q448K, pH 7.0, 30°C | Nicotiana tabacum | |
200 | - |
nitrite | wild-type, pH 7.0, 30°C | Nicotiana tabacum | |
227 | - |
nitrite | mutant M175G, pH 7.0, 30°C | Nicotiana tabacum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
[4Fe-4S]-center | - |
Nicotiana tabacum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
125 | - |
nitrite | mutant M175E, pH 7.0, 30°C | Nicotiana tabacum | |
134 | - |
nitrite | mutant M175GK pH 7.0, 30°C | Nicotiana tabacum | |
208 | - |
nitrite | mutant M175G, pH 7.0, 30°C | Nicotiana tabacum | |
332 | - |
nitrite | mutant K449Q, pH 7.0, 30°C | Nicotiana tabacum | |
341 | - |
nitrite | wild-type, pH 7.0, 30°C | Nicotiana tabacum | |
467 | - |
nitrite | wild-type, pH 7.0, 30°C | Nicotiana tabacum | |
473 | - |
nitrite | mutant Q448K, pH 7.0, 30°C | Nicotiana tabacum |