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Literature summary for 1.7.7.1 extracted from

  • Nakano, S.; Takahashi, M.; Sakamoto, A.; Morikawa, H.; Katayanagi, K.
    Structure-function relationship of assimilatory nitrite reductases from the leaf and root of tobacco based on high-resolution structures (2012), Protein Sci., 21, 383-395.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.7.7.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Nicotiana tabacum
expression in Escherichia coli Nicotiana tabacum

Crystallization (Commentary)

Crystallization (Comment) Organism
wild-type, to 1.25 A resolution and mutants Q448K, M175E, M175G, M175K to 2.0, 1.7. 1.7, 19 A resolution, respectively. The structure provides detailed geometries for the [4Fe–4S] cluster and the siroheme prosthetic groups Nicotiana tabacum

Protein Variants

Protein Variants Comment Organism
K449Q mutation does not affect enzymatic activity Nicotiana tabacum
M175E the conformation of the Gln47 and Lys91 side-chains changes significantly. Compared with the conformation in wild-type, the Gln47 side-chain rotates about 120 degrees and the amino group in the Lys91 side-chain moves toward the side-chain of residue 175 Nicotiana tabacum
M175G the conformation of the Gln47 and Lys91 side-chains changes significantly. Compared with the conformation in wild-type, the Gln47 side-chain rotates about 120 degrees and the amino group in the Lys91 side-chain moves toward the side-chain of residue 175 Nicotiana tabacum
M175K the conformation of the Gln47 and Lys91 side-chains changes significantly. Compared with the conformation in wild-type, the Gln47 side-chain rotates about 120 degrees and the amino group in the Lys91 side-chain moves toward the side-chain of residue 175 Nicotiana tabacum
Q448K mutation had only a negligible effect on the overall fold of the protein. In addition, the mutation did not affect the hydrogen bond interaction at the distal position of the siroheme Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.156
-
 nitrite wild-type, pH 7.0, 30°C Nicotiana tabacum
0.226
-
 nitrite mutant Q448K, pH 7.0, 30°C Nicotiana tabacum
0.277
-
 nitrite mutant K449Q, pH 7.0, 30°C Nicotiana tabacum
0.587
-
 nitrite wild-type, pH 7.0, 30°C Nicotiana tabacum
0.76
-
 nitrite mutant M175GK pH 7.0, 30°C Nicotiana tabacum
0.767
-
 nitrite mutant M175E, pH 7.0, 30°C Nicotiana tabacum
1.087
-
 nitrite mutant M175G, pH 7.0, 30°C Nicotiana tabacum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63700
-
 x * 63700, calculated Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum Q76KA9 isoform nii4
-
Nicotiana tabacum Q76KB0 isoform nii3
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Nicotiana tabacum
-
root
-
 Nicotiana tabacum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrite + reduced methyl viologen
-
 Nicotiana tabacum NH3 + H2O + oxidized methyl viologen
-
 ?

Subunits

Subunits Comment Organism
? x * 63700, calculated Nicotiana tabacum

Synonyms

Synonyms Comment Organism
aNiR
-
 Nicotiana tabacum
assimilatory nitrite reductase
-
 Nicotiana tabacum
nii3
-
 Nicotiana tabacum
nii4
-
 Nicotiana tabacum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
73
-
 nitrite wild-type, pH 7.0, 30°C Nicotiana tabacum
92
-
 nitrite mutant K449Q, pH 7.0, 30°C Nicotiana tabacum
96
-
 nitrite mutant M175E, pH 7.0, 30°C Nicotiana tabacum
102
-
 nitrite mutant M175GK pH 7.0, 30°C Nicotiana tabacum
107
-
 nitrite mutant Q448K, pH 7.0, 30°C Nicotiana tabacum
200
-
 nitrite wild-type, pH 7.0, 30°C Nicotiana tabacum
227
-
 nitrite mutant M175G, pH 7.0, 30°C Nicotiana tabacum

Cofactor

Cofactor Comment Organism Structure
[4Fe-4S]-center
-
 Nicotiana tabacum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
125
-
 nitrite mutant M175E, pH 7.0, 30°C Nicotiana tabacum
134
-
 nitrite mutant M175GK pH 7.0, 30°C Nicotiana tabacum
208
-
 nitrite mutant M175G, pH 7.0, 30°C Nicotiana tabacum
332
-
 nitrite mutant K449Q, pH 7.0, 30°C Nicotiana tabacum
341
-
 nitrite wild-type, pH 7.0, 30°C Nicotiana tabacum
467
-
 nitrite wild-type, pH 7.0, 30°C Nicotiana tabacum
473
-
 nitrite mutant Q448K, pH 7.0, 30°C Nicotiana tabacum