Cloned (Comment) | Organism |
---|---|
fusion with beta-galactosidase | Spinacia oleracea |
Protein Variants | Comment | Organism |
---|---|---|
C514S | almost complete loss of activity | Spinacia oleracea |
C518S | almost complete loss of activity | Spinacia oleracea |
G513A | increased Km for nitrite compared to the wild type enzyme | Spinacia oleracea |
G513E | loss of most of the activity | Spinacia oleracea |
G513V | loss of most of the activity | Spinacia oleracea |
G519A | 68% activity of wild type enzyme | Spinacia oleracea |
G519E | 8% activity of wild type enzyme | Spinacia oleracea |
G519V | 9% activity of wild type enzyme | Spinacia oleracea |
P515A | marginal loss of activity | Spinacia oleracea |
P515S | marginal loss of activity | Spinacia oleracea |
P515T | marginal loss of activity | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
nitrite | - |
Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
SDS-PAGE | Spinacia oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
homogeneity | Spinacia oleracea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
100 | - |
- |
Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrite + reduced ferredoxin | - |
Spinacia oleracea | ammonia + oxidized ferredoxin | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
siroheme | - |
Spinacia oleracea |