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Literature summary for 1.7.7.1 extracted from
- Ferredoxin:nitrite oxidoreductase from Chlorella. Purification and properties (1972), Biochim. Biophys. Acta, 276, 363-375.
General Stability
| General Stability | Organism |
|---|---|
| unstable in crude extracts and in solutions below pH 7 | [Chlorella] fusca |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 8-hydroxyquinoline | 7% inhibition at 5 mM | [Chlorella] fusca |  |
| CO | - |
[Chlorella] fusca | |
| cyanide | 90% inhibition at 1 mM | [Chlorella] fusca | |
| EDTA | - |
[Chlorella] fusca | |
| o-phenanthroline | 15% inhibition at 5 mM | [Chlorella] fusca | |
| p-chloromercuribenzoate | complete inhibition at 0.1 mM | [Chlorella] fusca | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | 2 atoms of iron probably bound in the chromophoric group | [Chlorella] fusca | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63000 | - |
gel filtration, SDS-PAGE | [Chlorella] fusca |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| [Chlorella] fusca | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
[Chlorella] fusca |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 51.7 | - |
- |
[Chlorella] fusca |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -24°C, 30% loss of activity within 4 months | [Chlorella] fusca |
| 4°C, 30% loss of activity within 3 weeks | [Chlorella] fusca |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrite + reduced ferredoxin | other electron donor: methyl viologen | [Chlorella] fusca | ammonia + oxidized ferredoxin | - |
? | |
| nitrite + reduced ferredoxin | enzyme can also catalyze the reduction of hydroxylamine to ammonia, at lower rate | [Chlorella] fusca | ammonia + oxidized ferredoxin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 63000 SDS-PAGE + mercaptoethanol | [Chlorella] fusca |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
enzyme in crude extract is rapidly inactivated below pH 7 | [Chlorella] fusca |
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Release 2023.1 (January 2023)
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