Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21. Expression and reconstitution method for NP7 that yields sufficient amounts of pure protein for extensive characterization | Rhodnius prolixus |
General Stability | Organism |
---|---|
NP7 is unstable when lyophilized | Rhodnius prolixus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20966 | - |
x * 20966, MALDI-TOF-MS | Rhodnius prolixus |
20969 | - |
x * 20969, calculated from sequence | Rhodnius prolixus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodnius prolixus | Q6PQK2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodnius prolixus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
saliva | - |
Rhodnius prolixus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | NP7 is unique in its ability to bind to negatively charged cell surfaces | Rhodnius prolixus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 20966, MALDI-TOF-MS | Rhodnius prolixus |
? | x * 20969, calculated from sequence | Rhodnius prolixus |
Synonyms | Comment | Organism |
---|---|---|
Nitrophorin 7 | - |
Rhodnius prolixus |
NP7 | - |
Rhodnius prolixus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme b | nitrophorins bind the heme b cofactor in the beta-barrel of their lipocalin fold, which is further anchored through a proximal histidine-Fe(III) bond. Ferriheme is not reduced by excess NO | Rhodnius prolixus |