Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NO | substrate inhibition at high concentratins | Pyrobaculum aerophilum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
integral to membrane | - |
Pyrobaculum aerophilum | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | qNOR contains two heme iron and one nonheme iron | Pyrobaculum aerophilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78800 | - |
1 * 78800, deduced from nucleotide sequence | Pyrobaculum aerophilum |
127000 | - |
gel filtration | Pyrobaculum aerophilum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NO + menaquinol | Pyrobaculum aerophilum | - |
N2O + H2O + menaquinone | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrobaculum aerophilum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
1% dodecyl maltoside, CM-Sepharose, hydroxyapatite | Pyrobaculum aerophilum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.28 | - |
at 54.5°C | Pyrobaculum aerophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NO + menaquinol | - |
Pyrobaculum aerophilum | N2O + H2O + menaquinone | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 75000-80000, SDS-PAGE, assuming that qNOR binds one dodecyl maltoside micelle of about 40000-50000 Da | Pyrobaculum aerophilum |
monomer | 1 * 78800, deduced from nucleotide sequence | Pyrobaculum aerophilum |
Synonyms | Comment | Organism |
---|---|---|
qNOR | - |
Pyrobaculum aerophilum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
93 | - |
qNOR in membranes | Pyrobaculum aerophilum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 93 | no activity detectable below 25°C | Pyrobaculum aerophilum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
no loss of activity after 24 h, qNOR in membrane | Pyrobaculum aerophilum |
56 | - |
50% loss of activity after 630 min, qNOR in membrane | Pyrobaculum aerophilum |
75 | - |
50% loss of activity after 231 min, qNOR in membrane | Pyrobaculum aerophilum |
100 | - |
50% loss of activity after 4 min, purified qNOR | Pyrobaculum aerophilum |
100 | - |
50% loss of activity after 86 min, qNOR in membrane | Pyrobaculum aerophilum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Pyrobaculum aerophilum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | approx. 50% of maximal activity at pH 5.0, almost no activity at pH 10.0 | Pyrobaculum aerophilum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
NO | 54.5°C, pH 7.0, qNOR in membrane | Pyrobaculum aerophilum | |
0.015 | - |
NO | 54.5°C, pH 7.0, purified qNOR | Pyrobaculum aerophilum |