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Literature summary for 1.7.5.1 extracted from
- Demethylmenaquinol is a substrate of Escherichia coli nitrate reductase A (NarGHI) and forms a stable semiquinone intermediate at the NarGHI quinol oxidation site (2015), Biochim. Biophys. Acta, 1847, 739-747 .
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Escherichia coli | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrite + demethylmenaquinone + H2O | Escherichia coli | - |
nitrate + demethylmenaquinol | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P09152 | NarG; MG1655 | - |
| Escherichia coli | P11349 | NarH; MG1655 | - |
| Escherichia coli | P11350 | NarI; MG1655 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | structure-function relationships of quinone reactivity | Escherichia coli | ? | - |
? | |
| nitrite + demethylmenaquinone + H2O | - |
Escherichia coli | nitrate + demethylmenaquinol | - |
? | |
| nitrite + demethylmenaquinone + H2O | endogeneous demethylmenasemiquinone (DMSK) intermediates are stabilized in the enzyme. DMSK is formed at the NarGHI QD quinol oxidation site | Escherichia coli | nitrate + demethylmenaquinol | - |
? | |
| nitrite + menadione + H2O | - |
Escherichia coli | nitrate + menadiol | - |
? | |
| nitrite + menaquinone + H2O | - |
Escherichia coli | nitrate + menaquinol | - |
? | |
| nitrite + naphthoquinone + H2O | - |
Escherichia coli | nitrate + naphthoquinol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NarG | - |
Escherichia coli |
| NarGHI | - |
Escherichia coli |
| narH | - |
Escherichia coli |
| NarI | - |
Escherichia coli |
| nitrate reductase A | - |
Escherichia coli |
| quinol:nitrate oxidoreductase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 1,4-Naphthoquinone | - |
Escherichia coli | |
| 2-methylnaphthalene-1,4-dione | - |
Escherichia coli | |
| demethylmenaquinone | DMKH2, endogeneous demethylmenasemiquinone (DMSK) intermediates are stabilized in the enzyme | Escherichia coli | |
| heme | - |
Escherichia coli | |
| vitamin K2 | - |
Escherichia coli | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | structure-function relationships of quinone reactivity. The NarGHI catalytic activity measured with the demethylmenaquinol (DMKH2) analogue 1,4-naphthoquinol is comparable to that measured using the corresponding methylated methylmenaquinol (MKH2) analogue menadiol, kinetics, overview | Escherichia coli |
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Release 2023.1 (January 2023)
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