Literature summary for 1.7.3.3 extracted from

Wang, J.; Zhang, L.; Rao, J.; Yang, L.; Yang, X.; Liao, F.
Design of Bacillus fastidious uricase mutants bearing long lagging phases before exponential decreases of activities under physiological conditions (2021), Protein J., 40, 765-775 .

Search for more literature for 1.7.3.3

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Metabacillus fastidiosus

Protein Variants

Protein Variants	Comment	Organism
V144A	the mutant has about 25% increase of the activity against wild type	Metabacillus fastidiosus
V144A/Y319R	the mutant has about 25% increase of the activity against wild type	Metabacillus fastidiosus
V144A/Y319R	the mutant shows an approximately 4 week lagging phase before the exponential activity decrease, an apparent half-life of activity nearly 3folds of mutant V144A, but comparable activity	Metabacillus fastidiosus
Y319R	the mutant shows wild type activity and has much stronger thermostability than the wild type enzyme	Metabacillus fastidiosus

Inhibitors

Inhibitors	Comment	Organism	Structure
oxonate	competitive inhibitor	Metabacillus fastidiosus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.23	-	Urate	mutant enzyme V144A, at pH 9.2 and 25°C	Metabacillus fastidiosus
0.24	-	Urate	mutant enzyme V144A, at pH 7.4 and 25°C	Metabacillus fastidiosus
0.247	-	Urate	wild type enzyme, at pH 7.4 and 25°C	Metabacillus fastidiosus
0.267	-	Urate	wild type enzyme, at pH 9.2 and 25°C	Metabacillus fastidiosus
0.28	-	Urate	mutant enzyme Y319R, at pH 9.2 and 25°C	Metabacillus fastidiosus
0.29	-	Urate	mutant enzyme Y319R, at pH 7.4 and 25°C	Metabacillus fastidiosus
0.293	-	Urate	mutant enzyme V144A/Y319R, at pH 9.2 and 25°C	Metabacillus fastidiosus
0.3	-	Urate	mutant enzyme V144A/Y319R, at pH 7.4 and 25°C	Metabacillus fastidiosus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
urate + O2 + H2O	Metabacillus fastidiosus	-	5-hydroxyisourate + H2O2	-	?

Organism

Organism	UniProt	Comment	Textmining
Metabacillus fastidiosus	C5HDG5	-	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE cellulose column chromatography	Metabacillus fastidiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
9	-	wild type enzyme, at pH 9.2 and 25°C	Metabacillus fastidiosus
9.2	-	mutant enzyme Y319R, at pH 9.2 and 25°C	Metabacillus fastidiosus
11.3	-	mutant enzyme V144A/Y319R, at pH 9.2 and 25°C	Metabacillus fastidiosus
11.4	-	mutant enzyme V144A, at pH 9.2 and 25°C	Metabacillus fastidiosus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
urate + O2 + H2O	-	Metabacillus fastidiosus	5-hydroxyisourate + H2O2	-	?

Subunits

Subunits	Comment	Organism
homotetramer	4 *35 000, SDS-PAGE	Metabacillus fastidiosus

Synonyms

Synonyms	Comment	Organism
uricase	-	Metabacillus fastidiosus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00035	-	oxonate	mutant enzyme V144A/Y319R, at pH 7.4 and 25°C	Metabacillus fastidiosus
0.00042	-	oxonate	mutant enzyme Y319R, at pH 7.4 and 25°C	Metabacillus fastidiosus
0.00045	-	oxonate	mutant enzyme V144A, at pH 7.4 and 25°C	Metabacillus fastidiosus
0.00052	-	oxonate	wild type enzyme, at pH 7.4 and 25°C	Metabacillus fastidiosus
0.0042	-	oxonate	mutant enzyme Y319R, at pH 9.2 and 25°C	Metabacillus fastidiosus
0.0049	-	oxonate	mutant enzyme V144A, at pH 9.2 and 25°C	Metabacillus fastidiosus
0.0049	-	oxonate	mutant enzyme V144A/Y319R, at pH 9.2 and 25°C	Metabacillus fastidiosus
0.0056	-	oxonate	wild type enzyme, at pH 9.2 and 25°C	Metabacillus fastidiosus

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Release 2023.1 (January 2023)