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Literature summary for 1.7.3.3 extracted from

  • Mirzaeinia, S.; Pazhang, M.; Imani, M.; Chaparzadeh, N.; Amani-Ghadim, A.
    Improving the stability of uricase from Aspergillus flavus by osmolytes Use of response surface methodology for optimization of the enzyme stability (2020), Process Biochem., 94, 86-98 .
No PubMed abstract available
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Aspergillus flavus

General Stability

General Stability Organism
the stability of the enzyme increases up to 4.9fold in the presence of 12% (w/v) of glycerol and sucrose. The effect of sorbitol on enzyme stability is negligible in the presence of glycerol and sucrose. In the presence of 20% (w/v) glycerol, sorbitol, and sucrose, the enzyme has the highest stability Aspergillus flavus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
135000
-
 gel filtration Aspergillus flavus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
urate + O2 + H2O Aspergillus flavus
-
 5-hydroxyisourate + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus flavus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography, and Superdex 200 gel filtration Aspergillus flavus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urate + O2 + H2O
-
 Aspergillus flavus 5-hydroxyisourate + H2O2
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 34000, SDS-PAGE Aspergillus flavus

Synonyms

Synonyms Comment Organism
Uox
-
 Aspergillus flavus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 70 the enzyme remains stable (more than 90% activity) for 30 min at 0-20°C. The activity drops to about 60, 50, 40, less than 30, and less than 20% after 30 min at 30, 40, 50, 60, and 70°C, respectively. At 40°C, the enzyme shows about 80% activity after 5 min, about 65% activity after 10 min, about 50% activity after 20-40 min, and about 30% activity after 50-60 min Aspergillus flavus