Literature summary for 1.7.3.3 extracted from

Shi, Y.; Wang, T.; Zhou, X.E.; Liu, Q.F.; Jiang, Y.; Xu, H.E.
Structure-based design of a hyperthermostable AgUricase for hyperuricemia and gout therapy (2019), Acta Pharmacol. Sin., 40, 1364-1372 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Arthrobacter globiformis

Crystallization (Commentary)

Crystallization (Comment)	Organism
mutant enzyme K12C/E286C, sitting drop vapor diffusion method, using 12% (w/v) polyethylene glycol 3350 and 0.1 M sodium malonate (pH 5.0)	Arthrobacter globiformis

Protein Variants

Protein Variants	Comment	Organism
K12C/E286C	the mutations introduce disulfide bonds between the subunits and markedly increase the melting temperature (from 61 to 75°C) of the enzyme compared with wild type	Arthrobacter globiformis
S296C/C302S	the mutations introduce disulfide bonds between the subunits and markedly increase the melting temperature (from 61 to 70°C) of the enzyme compared with wild type	Arthrobacter globiformis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.1045	-	Urate	mutant enzyme K12C/E286C, at pH 7.4, temperature not specified in the publication	Arthrobacter globiformis
0.1268	-	Urate	mutant enzyme S296C/C302S, at pH 7.4, temperature not specified in the publication	Arthrobacter globiformis
0.1403	-	Urate	wild type enzyme, at pH 7.4, temperature not specified in the publication	Arthrobacter globiformis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
120000	-	gel filtration	Arthrobacter globiformis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
urate + O2 + H2O	Arthrobacter globiformis	-	5-hydroxyisourate + H2O2	-	?

Organism

Organism	UniProt	Comment	Textmining
Arthrobacter globiformis	D0VWQ1	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel resin column chromatography and Superdex 200 gel filtration	Arthrobacter globiformis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
urate + O2 + H2O	-	Arthrobacter globiformis	5-hydroxyisourate + H2O2	-	?

Subunits

Subunits	Comment	Organism
homotetramer	4 * 34000, SDS-PAGE	Arthrobacter globiformis

Synonyms

Synonyms	Comment	Organism
uricase	-	Arthrobacter globiformis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
61	-	melting temperature	Arthrobacter globiformis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
5.8	-	Urate	mutant enzyme K12C/E286C, at pH 7.4, temperature not specified in the publication	Arthrobacter globiformis
5.89	-	Urate	mutant enzyme S296C/C302S, at pH 7.4, temperature not specified in the publication	Arthrobacter globiformis
6.64	-	Urate	wild type enzyme, at pH 7.4, temperature not specified in the publication	Arthrobacter globiformis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
46.4	-	Urate	mutant enzyme S296C/C302S, at pH 7.4, temperature not specified in the publication	Arthrobacter globiformis
47.3	-	Urate	wild type enzyme, at pH 7.4, temperature not specified in the publication	Arthrobacter globiformis
55.5	-	Urate	mutant enzyme K12C/E286C, at pH 7.4, temperature not specified in the publication	Arthrobacter globiformis

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Release 2023.1 (January 2023)