Any feedback?
Literature summary for 1.7.3.3 extracted from
- Efficient purification of a recombinant tag-free thermostable Kluyveromyces marxianus uricase by pH-induced self-cleavage of intein and expression in Escherichia coli (2018), 3 Biotech, 8, 400 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetone | 108.42% activity at 20% (v/v) | Kluyveromyces marxianus |  |
| DMSO | 103.19% activity at 20% (v/v) | Kluyveromyces marxianus | |
| EDTA | 110.57% activity at 20% (v/v) | Kluyveromyces marxianus | |
| n-hexane | 102.21% activity at 20% (v/v) | Kluyveromyces marxianus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Kluyveromyces marxianus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 62.98% residual activity at 20% (v/v) | Kluyveromyces marxianus | |
| acetaldehyde | complete inhibition at 20% (v/v) | Kluyveromyces marxianus | |
| Ca2+ | 95.32% residual activity at 1 mM | Kluyveromyces marxianus | |
| Cu2+ | 19.82% residual activity at 1 mM | Kluyveromyces marxianus | |
| dithiothreitol | 94.93% residual activity at 20% (v/v) | Kluyveromyces marxianus | |
| ethanol | 71.97% residual activity at 20% (v/v) | Kluyveromyces marxianus | |
| Fe2+ | 6.07% residual activity at 1 mM | Kluyveromyces marxianus | |
| Fe3+ | complete inhibition at 1 mM | Kluyveromyces marxianus | |
| H2O2 | 68.68% residual activity at 0.02 mM | Kluyveromyces marxianus | |
| K+ | 88.69% residual activity at 1 mM | Kluyveromyces marxianus | |
| methanol | 84.55% residual activity at 20% (v/v) | Kluyveromyces marxianus | |
| Mg2+ | 87.99% residual activity at 1 mM | Kluyveromyces marxianus | |
| Na+ | 90.92% residual activity at 1 mM | Kluyveromyces marxianus | |
| Ni2+ | 84.77% residual activity at 1 mM | Kluyveromyces marxianus | |
| PEG4000 | 68.04% residual activity at 20% (v/v) | Kluyveromyces marxianus | |
| Tween 80 | 95.26% residual activity at 20% (v/v) | Kluyveromyces marxianus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0676 | - |
Urate | at pH 9.0 and 42°C | Kluyveromyces marxianus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urate + O2 + H2O | Kluyveromyces marxianus | - |
5-hydroxyisourate + H2O2 | - |
? | |
| urate + O2 + H2O | Kluyveromyces marxianus NBRC 1777 | - |
5-hydroxyisourate + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Kluyveromyces marxianus | - |
- |
- |
| Kluyveromyces marxianus NBRC 1777 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| RAC | Kluyveromyces marxianus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.36 | - |
soluble cell lysate, at pH 9.0 and 42°C | Kluyveromyces marxianus |
| 50.54 | - |
after 9.43fold purification, at pH 9.0 and 42°C | Kluyveromyces marxianus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urate + O2 + H2O | - |
Kluyveromyces marxianus | 5-hydroxyisourate + H2O2 | - |
? | |
| urate + O2 + H2O | - |
Kluyveromyces marxianus NBRC 1777 | 5-hydroxyisourate + H2O2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| uricase | - |
Kluyveromyces marxianus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
- |
Kluyveromyces marxianus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 45 | the retained enzymatic activity at 40 and 45°C is 91.63% and 93.32%, respectively | Kluyveromyces marxianus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
the enzyme retains 79.75% of activity when incubated at 40°C for 90 h. After incubation of the enzyme for 15 min at 40 and 45°C, the retained activities are 95.55% and 74.55%, respectivelyThe retained activity decreases significantly when the incubation temperature is above 55°C | Kluyveromyces marxianus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 32.74 | - |
Urate | at pH 9.0 and 42°C | Kluyveromyces marxianus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Kluyveromyces marxianus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | the enzyme activity remains stable after 15 min at pH 6.0-11.0 (4, 25 and 37°C) | Kluyveromyces marxianus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 484.32 | - |
Urate | at pH 9.0 and 42°C | Kluyveromyces marxianus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
