Application | Comment | Organism |
---|---|---|
analysis | uric acid can be detected in human sera with the enzyme with none of the tested uric acid analogs being a competitive substrate | Cytobacillus firmus |
General Stability | Organism |
---|---|
stable against oxidants and SDS | Cytobacillus firmus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,9-Dimethyl-1,10-phenanthroline | 0.1 mM, 64% residual activity | Cytobacillus firmus | |
Cu2+ | 1 mM, 15% residual activity | Cytobacillus firmus | |
EDTA | 20 mM, 1% residual activity | Cytobacillus firmus | |
Fe3+ | 1 mM, 36% residual activity | Cytobacillus firmus | |
H2O2 | purified uricase retains 72% of its original activity after incubation with 0.5% H2O2 for 6 h | Cytobacillus firmus | |
Hg2+ | 1 mM, 2% residual activity | Cytobacillus firmus | |
additional information | not inhibitory: Tween-20 or Triton X-100 at 0.5% w/v | Cytobacillus firmus | |
o-phenanthroline | 2 mM, 4% residual activity | Cytobacillus firmus | |
SDS | purified uricase retains 82% of its original activity after incubation with 0.5% SDS for 6 h | Cytobacillus firmus | |
Tween-80 | 0.5% w/v, 61% residual activity | Cytobacillus firmus | |
Zn2+ | 1 mM, 22% residual activity | Cytobacillus firmus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.079 | - |
Urate | pH 8.0, 40°C | Cytobacillus firmus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 1 mM, 138% of initial activity | Cytobacillus firmus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cytobacillus firmus | - |
- |
- |
Cytobacillus firmus DWD-33 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cytobacillus firmus |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urate + O2 + H2O | - |
Cytobacillus firmus | 5-hydroxyisourate + H2O2 | - |
? | |
urate + O2 + H2O | - |
Cytobacillus firmus DWD-33 | 5-hydroxyisourate + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33500, SDS-PAGE | Cytobacillus firmus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Cytobacillus firmus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
stable up to | Cytobacillus firmus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21800 | - |
Urate | pH 8.0, 40°C | Cytobacillus firmus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Cytobacillus firmus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | - |
Cytobacillus firmus |
Organism | Comment | Expression |
---|---|---|
Cytobacillus firmus | highest expression yields are obtained in medium containing 0.8% maltose and 1.2% soybean powder as carbon and nitrogen sources, respectively | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
276000 | - |
Urate | pH 8.0, 40°C | Cytobacillus firmus |