Application | Comment | Organism |
---|---|---|
synthesis | at optimized growth parameters, the crude preparation shows uricase activity of 13.42 U/ml | Pseudomonas aeruginosa |
synthesis | at optimized growth parameters, the crude preparation shows uricase activity of 17.7 U/ml | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, about 50% inhibition | Pseudomonas aeruginosa | |
Mg2+ | 1 mM, about 30% inhibition; 1 mM, about 50% inhibition | Pseudomonas aeruginosa | |
Ni2+ | 1 mM, about 50% inhibition; 1 mM, about 70% inhibition | Pseudomonas aeruginosa | |
Zn2+ | 1 mM, about 35% inhibition; 1 mM, about 50% inhibition | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
Urate | Km value for degradation of urate crystals is 7 microg/ml, 30°C, pH not specified in the publication | Pseudomonas aeruginosa | |
additional information | - |
Urate | Km value for degradation of urate crystals is 9 microg/ml, 30°C, pH not specified in the publication | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Pseudomonas aeruginosa 5Y2 | - |
- |
- |
Pseudomonas aeruginosa Ph3 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urate + O2 + H2O | - |
Pseudomonas aeruginosa | 5-hydroxyisourate + H2O2 | - |
? | |
urate + O2 + H2O | - |
Pseudomonas aeruginosa 5Y2 | 5-hydroxyisourate + H2O2 | - |
? | |
urate + O2 + H2O | - |
Pseudomonas aeruginosa Ph3 | 5-hydroxyisourate + H2O2 | - |
? |
Organism | Comment | Expression |
---|---|---|
Pseudomonas aeruginosa | expression is induced in presence of urate | up |
Pseudomonas aeruginosa | expression is induced in presence of urate, with substrate inhibition of production above 8.9 mM | up |