BRENDA - Enzyme Database
show all sequences of 1.7.3.3

Large crystal growth by thermal control allows combined X-ray and neutron crystallographic studies to elucidate the protonation states in Aspergillus flavus urate oxidase

Oksanen, E.; Blakeley, M.P.; Bonnete, F.; Dauvergne, M.T.; Dauvergne, F.; Budayova-Spano, M.; J. R. Soc. Interface 6 Suppl 5, 599-610 (2009)

Data extracted from this reference:

Application
Application
Commentary
Organism
medicine
urate oxidase is used to reduce toxic urate accumulation during chemotherapy
Aspergillus flavus
Crystallization (Commentary)
Crystallization (Commentary)
Organism
crystals of about a few tens of micrometres in size, which is nucleated previously in crystallization batch containing 5% PEG 8000, 100 mM NaCl, 8 mg/ml uox-substrate complex and 100 mM Tris-HCl pH 8.5, are used as seeds and their size and quality are further improved using a temperature-control device, large crystals of Uox, co-crystallized with its substrates analogues 8-azaxanthine, 9-methyluric acid or the natural substrate in the presence of cyanide (0.5-2 mg/ml), and soaks with the natural substrate in the absence of cyanide, diffracting to high resolutions are obtained, in the presence of different inhibitors, the crystal form of Uox has a body-centred orthorhombic symmetry and one of the largest primitive unit-cell volumes (a: 80 A, b: 96 A, c: 106 A)
Aspergillus flavus
Inhibitors
Inhibitors
Commentary
Organism
Structure
8-Azaxanthine
substrate analogue binds in the active site of the enzyme
Aspergillus flavus
9-methyluric acid
substrate analogue
Aspergillus flavus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
137000
-
for the homotetramer, determined by neutron crystallographic analysis
Aspergillus flavus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
urate + O2 + H2O
Aspergillus flavus
purine metabolism
5-hydroxyisourate + H2O2
a metastable intermediate, which is further degrades to allantoin
-
?
Organism
Organism
UniProt
Commentary
Textmining
Aspergillus flavus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
commercial preparation
-
Aspergillus flavus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
urate + O2 + H2O
purine metabolism
699832
Aspergillus flavus
5-hydroxyisourate + H2O2
a metastable intermediate, which is further degrades to allantoin
-
-
?
Subunits
Subunits
Commentary
Organism
homotetramer
determined by X-ray crystallography, formed by crystallographic symmetry operations
Aspergillus flavus
Synonyms
Synonyms
Commentary
Organism
Uox
-
Aspergillus flavus
Urate oxidase
-
Aspergillus flavus
Application (protein specific)
Application
Commentary
Organism
medicine
urate oxidase is used to reduce toxic urate accumulation during chemotherapy
Aspergillus flavus
Crystallization (Commentary) (protein specific)
Crystallization
Organism
crystals of about a few tens of micrometres in size, which is nucleated previously in crystallization batch containing 5% PEG 8000, 100 mM NaCl, 8 mg/ml uox-substrate complex and 100 mM Tris-HCl pH 8.5, are used as seeds and their size and quality are further improved using a temperature-control device, large crystals of Uox, co-crystallized with its substrates analogues 8-azaxanthine, 9-methyluric acid or the natural substrate in the presence of cyanide (0.5-2 mg/ml), and soaks with the natural substrate in the absence of cyanide, diffracting to high resolutions are obtained, in the presence of different inhibitors, the crystal form of Uox has a body-centred orthorhombic symmetry and one of the largest primitive unit-cell volumes (a: 80 A, b: 96 A, c: 106 A)
Aspergillus flavus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
8-Azaxanthine
substrate analogue binds in the active site of the enzyme
Aspergillus flavus
9-methyluric acid
substrate analogue
Aspergillus flavus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
137000
-
for the homotetramer, determined by neutron crystallographic analysis
Aspergillus flavus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
urate + O2 + H2O
Aspergillus flavus
purine metabolism
5-hydroxyisourate + H2O2
a metastable intermediate, which is further degrades to allantoin
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
commercial preparation
-
Aspergillus flavus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
urate + O2 + H2O
purine metabolism
699832
Aspergillus flavus
5-hydroxyisourate + H2O2
a metastable intermediate, which is further degrades to allantoin
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
determined by X-ray crystallography, formed by crystallographic symmetry operations
Aspergillus flavus
Other publictions for EC 1.7.3.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)