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Literature summary for 1.7.3.3 extracted from

  • Suzuki, K.; Sakasegawa, S.I.; Misaki, H.; Sugiyama, M.
    Molecular cloning and expression of uricase gene from Arthrobacter globiformis in Escherichia coli and characterization of the gene product (2004), J. Biosci. Bioeng., 98, 153-158.
    View publication on PubMed
Search for more literature for 1.7.3.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, Escherichia coli harboring pUOD1 produces 20fold higher uricase than the original Arthrobacter strain, even without an inducer Arthrobacter globiformis

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ enzyme contains copper, inhibited by excessive addition of Cu2+, 98% inhibition by 1 mM CuCl2, 97% inhibition by 1 mM CuSO4 Arthrobacter globiformis
additional information no inhibition by 1 mM Fe(ClO4)3 or 1 mM AgNO3 Arthrobacter globiformis
ZnCl2 1 mM, 91% inhibition Arthrobacter globiformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.075
-
 Urate pH 7.0, 37°C Arthrobacter globiformis

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Arthrobacter globiformis 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ enzyme contains copper, inhibited by excessive addition of Cu2+ Arthrobacter globiformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 4 * 33000, SDS-PAGE Arthrobacter globiformis
33858
-
 4 * 33858, calculation from nucleotide sequence Arthrobacter globiformis
117000
-
 gel filtration Arthrobacter globiformis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
urate + O2 + H2O Arthrobacter globiformis enzyme production is induced by addition of uric acid to the culture medium 5-hydroxyisourate + H2O2
-
 ?
urate + O2 + H2O Arthrobacter globiformis FERM BP-360 enzyme production is induced by addition of uric acid to the culture medium 5-hydroxyisourate + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Arthrobacter globiformis
-
 FERM BP-360
-
Arthrobacter globiformis FERM BP-360
-
 FERM BP-360
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urate + O2 + H2O enzyme production is induced by addition of uric acid to the culture medium Arthrobacter globiformis 5-hydroxyisourate + H2O2
-
 ?
urate + O2 + H2O enzyme production is induced by addition of uric acid to the culture medium Arthrobacter globiformis FERM BP-360 5-hydroxyisourate + H2O2
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 33000, SDS-PAGE Arthrobacter globiformis
tetramer 4 * 33858, calculation from nucleotide sequence Arthrobacter globiformis

Synonyms

Synonyms Comment Organism
Urate oxidase
-
 Arthrobacter globiformis
uricase
-
 Arthrobacter globiformis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 10 min, stable Arthrobacter globiformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
 Arthrobacter globiformis

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.5 11 stable Arthrobacter globiformis

pI Value

Organism Comment pI Value Maximum pI Value
Arthrobacter globiformis isoelectric focusing, pH gradient 3.5-9.5
-
 4.96