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Literature summary for 1.7.3.3 extracted from
- Purification and properties of uricase from Enterobacter cloacae (1980), Agric. Biol. Chem., 44, 2811-2815.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Enterobacter cloacae |  |
| CN- | - |
Enterobacter cloacae | |
| Co2+ | - |
Enterobacter cloacae | |
| Cu2+ | - |
Enterobacter cloacae | |
| N-ethylmaleimide | - |
Enterobacter cloacae | |
| p-chloromercuribenzoate | - |
Enterobacter cloacae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.5 | - |
uric acid | - |
Enterobacter cloacae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell associated | - |
Enterobacter cloacae | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 105000 | - |
gel filtration | Enterobacter cloacae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urate + O2 + H2O | Enterobacter cloacae | - |
5-hydroxyisourate + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterobacter cloacae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Enterobacter cloacae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.6 | - |
- |
Enterobacter cloacae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urate + O2 + H2O | - |
Enterobacter cloacae | 5-hydroxyisourate + H2O2 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Enterobacter cloacae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | 20°C: about 60% of activity maximum | Enterobacter cloacae |
| 20 | 50 | 50°C: about 50% of activity maximum | Enterobacter cloacae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
30 min, stable below | Enterobacter cloacae |
| 60 | - |
30 min, about 40% loss of activity | Enterobacter cloacae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Enterobacter cloacae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 11 | pH 7: about 50% of activity maximum, pH 11: about 40% of activity maximum | Enterobacter cloacae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
45°C, 30 min, 40% loss of activity | Enterobacter cloacae |
| 8 | 9 | 45°C, 30 min, stable | Enterobacter cloacae |
| 10 | - |
45°C, 30 min, 30% loss of activity | Enterobacter cloacae |
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