Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.7.3.1 extracted from

  • Gadda, G.; Fitzpatrick, P.F.
    Iso-mechanism of nitroalkane oxidase: 1. Inhibition studies and activation by imidazole (2000), Biochemistry, 39, 1400-1405.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
imidazole unprotonated imidazole causes a 5fold increase in maximal velocity Fusarium oxysporum

Inhibitors

Inhibitors Comment Organism Structure
acetaldehyde noncompetitive inhibitor versus nitroethane due to formation of a dead-end complex between the oxidized enzyme and the product, competitive inhibitor versus oxygen Fusarium oxysporum
Butyraldehyde noncompetitive inhibitor versus nitroethane due to formation of a dead-end complex between the oxidized enzyme and the product Fusarium oxysporum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
O2
-
Fusarium oxysporum
3.3
-
nitroethane
-
Fusarium oxysporum

Organism

Organism UniProt Comment Textmining
Fusarium oxysporum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-nitrobutane + O2
-
Fusarium oxysporum butyraldehyde + HNO2
-
?
1-nitrohexane + O2
-
Fusarium oxysporum hexanaldehyde + HNO2
-
?
1-nitropentane + O2
-
Fusarium oxysporum pentanaldehyde + HNO2
-
?
1-nitropropane + O2
-
Fusarium oxysporum propionaldehyde + HNO2
-
?
2-nitropropane + O2
-
Fusarium oxysporum acetone + HNO2
-
?
nitroethane + O2 at pH 7, bi-ter ping-pong mechanism with oxygen reacting with the free reduced enzyme after release of the aldehyde product Fusarium oxysporum acetaldehyde + HNO2
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information
-
Fusarium oxysporum