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Literature summary for 1.7.2.6 extracted from

  • Zhao, B.; Ran, X.C.; An, Q.; Huang, Y.S.; Lv, Q.H.; Dan, Q.
    N2O production from hydroxylamine oxidation and corresponding hydroxylamine oxidoreductase involved in a heterotrophic nitrifier A. faecalis strain NR (2019), Bioprocess Biosyst. Eng., 42, 1983-1992 .
    View publication on PubMed
Search for more literature for 1.7.2.6

Inhibitors

Inhibitors Comment Organism Structure
Mn2+ strong inhibition at 3.75 mM Alcaligenes faecalis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the addition of 3.75 mM Ca2+ promotes the enzyme activity Alcaligenes faecalis
additional information not affected by Fe2+, Zn2+ and Mg2+ Alcaligenes faecalis

Organism

Organism UniProt Comment Textmining
Alcaligenes faecalis
-
-
-
Alcaligenes faecalis NR
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially purified by ammonium sulfate precipitation and DEAE column chromatography Alcaligenes faecalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000903
-
 with NAD+ as electron acceptor, at pH 8.0 and 30°C Alcaligenes faecalis
0.001546
-
 with 2,6-dichlorophenolindophenol as electron acceptor, at pH 8.0 and 30°C Alcaligenes faecalis
0.001662
-
 with phenazine methosulfate as electron acceptor, at pH 8.0 and 30°C Alcaligenes faecalis
0.00201
-
 with horse heart cytochrome c as electron acceptor, at pH 8.0 and 30°C Alcaligenes faecalis
0.02375
-
 cell-free extract, with ferricyanide as electron acceptor, at pH 8.0 and 30°C Alcaligenes faecalis
0.09204
-
 after 3.9fold purification, with ferricyanide as electron acceptor, at pH 8.0 and 30°C Alcaligenes faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hydroxylamine + H2O + 2,6-dichlorophenolindophenol very low activity Alcaligenes faecalis nitric oxide + reduced 2,6-dichlorophenolindophenol + H+
-
 ?
hydroxylamine + H2O + 2,6-dichlorophenolindophenol very low activity Alcaligenes faecalis NR nitric oxide + reduced 2,6-dichlorophenolindophenol + H+
-
 ?
hydroxylamine + H2O + ferricyanide best electron acceptor Alcaligenes faecalis nitric oxide + ferrocyanide + H+
-
 ?
hydroxylamine + H2O + ferricyanide best electron acceptor Alcaligenes faecalis NR nitric oxide + ferrocyanide + H+
-
 ?
hydroxylamine + H2O + horse heart cytochrome c very low activity Alcaligenes faecalis nitric oxide + reduced horse heart cytochrome c + H+
-
 ?
hydroxylamine + H2O + horse heart cytochrome c very low activity Alcaligenes faecalis NR nitric oxide + reduced horse heart cytochrome c + H+
-
 ?
hydroxylamine + H2O + NAD+ very low activity Alcaligenes faecalis nitric oxide + NADH + H+
-
 ?
hydroxylamine + H2O + NAD+ very low activity Alcaligenes faecalis NR nitric oxide + NADH + H+
-
 ?
hydroxylamine + H2O + phenazine methosulfate very low activity, in the presence of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide Alcaligenes faecalis nitric oxide + reduced phenazine methosulfate + H+
-
 ?
hydroxylamine + H2O + phenazine methosulfate very low activity, in the presence of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide Alcaligenes faecalis NR nitric oxide + reduced phenazine methosulfate + H+
-
 ?

Subunits

Subunits Comment Organism
? x * 25000, SDS-PAGE Alcaligenes faecalis

Synonyms

Synonyms Comment Organism
HAO
-
 Alcaligenes faecalis
hydroxylamine oxidoreductase
-
 Alcaligenes faecalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Alcaligenes faecalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Alcaligenes faecalis