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Literature summary for 1.7.2.5 extracted from

  • Tosha, T.; Shiro, Y.
    Crystal structures of nitric oxide reductases provide key insights into functional conversion of respiratory enzymes (2013), IUBMB Life, 65, 217-226 .
    View publication on PubMed
Search for more literature for 1.7.2.5

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure analysis, PDB ID 3O0R Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Pseudomonas aeruginosa 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ an iron-containing enzyme. Heme Fe–nonheme metal distances in the enzyme is 3.8 A Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrous oxide + 2 ferricytochrome c + H2O Pseudomonas aeruginosa
-
 2 nitric oxide + 2 ferrocytochrome c + 2 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q59647 AND Q59646 large subunit B and small subunit C encoded by genes norB and norC
-

Reaction

Reaction Comment Organism Reaction ID
nitrous oxide + 2 ferricytochrome c + H2O = 2 nitric oxide + 2 ferrocytochrome c + 2 H+ proton transfer by a trans-mechanism, not by cis-heme b3 mechanism,analysis, overview Pseudomonas aeruginosa
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrous oxide + 2 ferricytochrome c + H2O
-
 Pseudomonas aeruginosa 2 nitric oxide + 2 ferrocytochrome c + 2 H+
-
 ?

Subunits

Subunits Comment Organism
More structure analysis of Pseudomonas aeruginosa cNOR shows that three conserved glutamate residues locate at the close proximity to the active site and forms a small hydrophilic cavity Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
cNOR
-
 Pseudomonas aeruginosa
membrane-integrated nitric oxide reductase
-
 Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
cytochrome c heme containing Pseudomonas aeruginosa

General Information

General Information Comment Organism
additional information structure-based molecular dynamics simulation, overview. Active site located essential residue Glu211 of cNOR. Three conserved glutamate residues locate at the close proximity to the active site and forms a small hydrophilic cavity Pseudomonas aeruginosa
physiological function membrane-integrated nitric oxide reductase (NOR) is an iron-containing enzyme involved in the denitrification process and catalyzes the reduction of nitric oxide (NO) to nitrous oxide (N2O) with two protons and two electrons via cleavage of the N-O bond and concomitant formation of the N-N bond. No proton transfer pathway occurs from the inside of the membrane, but via two water channels, involving residue Asp198 in channel 1 of Pseudomonas aeruginosa cNOR, channel structure analysis, overview Pseudomonas aeruginosa