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Literature summary for 1.7.2.5 extracted from
- Formation of the N-N bond from nitric oxide by a membrane-bound cytochrome bc complex of nitrate-respiring (denitrifying) Pseudomonas stutzeri (1989), J. Bacteriol., 171, 3288-3297.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| n-octyl-beta-D-glucopyranoside | 340% activity | Pseudomonas stutzeri |  |
| n-octyl-beta-D-thioglucopyranoside | 340% activity | Pseudomonas stutzeri | |
| Soybean phospholipids | 340% activity | Pseudomonas stutzeri |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| KCN | - |
Pseudomonas stutzeri | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
phenazine methosulfate | - |
Pseudomonas stutzeri | |
| 0.06 | - |
NO | - |
Pseudomonas stutzeri | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | - |
Pseudomonas stutzeri | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 nitric oxide + reduced acceptor | Pseudomonas stutzeri | - |
nitrous oxide + acceptor + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas stutzeri | - |
ZoBell strain, ATCC 14405 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas stutzeri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.8 | - |
- |
Pseudomonas stutzeri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 nitric oxide + reduced acceptor | - |
Pseudomonas stutzeri | nitrous oxide + acceptor + H2O | - |
? | |
| 2 nitric oxide + reduced acceptor | ascorbate/phenazine methosulfate as electron donors | Pseudomonas stutzeri | nitrous oxide + acceptor + H2O | - |
? | |
| N2O + phenazine methosulfate + H2O | - |
Pseudomonas stutzeri | NO + reduced phenazine methosulfate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | - |
membrane-bound activity | Pseudomonas stutzeri |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Pseudomonas stutzeri | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.6 | - |
KCN | - |
Pseudomonas stutzeri | |
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