Literature summary for 1.7.2.4 extracted from

Fujita, K.; Hirasawa-Fujita, M.; Brown, D.E.; Obara, Y.; Ijima, F.; Kohzuma, T.; Dooley, D.M.
Direct electron transfer from pseudoazurin to nitrous oxide reductase in catalytic N2O reduction (2012), J. Inorg. Biochem., 115, 163-173.

Search for more literature for 1.7.2.4

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0138	-	reduced benzyl viologen	pH 7.1, temperature not specified in the publication	Achromobacter cycloclastes
0.0191	-	N2O	pH 7.1, temperature not specified in the publication	Achromobacter cycloclastes
0.0288	-	reduced pseudoazurin	pH 7.1, temperature not specified in the publication	Achromobacter cycloclastes

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
N2O + reduced pseudoazurin	Achromobacter cycloclastes	blue copper electron-transfer protein pseudoazurin is capable of mediating electron transfer to the nitrous oxide reductase. Pseudoazurin binds near copper site CuA, with parameters consistent with the formation of a transient, weakly-bound complex. Pseudoazurin a strong candidate for the physiological electron donor to th enzyme	N2 + oxidized pseudoazurin	-	?

Organism

Organism	UniProt	Comment	Textmining
Achromobacter cycloclastes	P94127	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N2O + reduced benzyl viologen	-	Achromobacter cycloclastes	N2 + oxidized benzyl viologen	-	?
N2O + reduced pseudoazurin	blue copper electron-transfer protein pseudoazurin is capable of mediating electron transfer to the nitrous oxide reductase. Pseudoazurin binds near copper site CuA, with parameters consistent with the formation of a transient, weakly-bound complex. Pseudoazurin a strong candidate for the physiological electron donor to th enzyme	Achromobacter cycloclastes	N2 + oxidized pseudoazurin	-	?

Synonyms

Synonyms	Comment	Organism
NosZ	-	Achromobacter cycloclastes

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
89.3	-	reduced pseudoazurin	pH 7.1, temperature not specified in the publication	Achromobacter cycloclastes
162.9	-	reduced benzyl viologen	pH 7.1, temperature not specified in the publication	Achromobacter cycloclastes

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Release 2023.1 (January 2023)