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Literature summary for 1.7.2.4 extracted from
- Anaerobic purification, characterization and preliminary mechanistic study of recombinant nitrous oxide reductase from Achromobacter cycloclastes (2007), J. Inorg. Biochem., 101, 1836-1844.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the gene encoding the enzyme in the nos cluster is cloned from a genomic library | Achromobacter cycloclastes |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady-state kinetics | Achromobacter cycloclastes |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | the enzyme contains average 4.5 Cu and 1.2 S per monomer | Achromobacter cycloclastes |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrous oxide + reduced methyl viologen | Achromobacter cycloclastes | - |
nitrogen + H2O + methyl viologen | - |
? | |
| nitrous oxide + reduced methyl viologen | Achromobacter cycloclastes IAM1013 | - |
nitrogen + H2O + methyl viologen | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Achromobacter cycloclastes | P94127 | - |
- |
| Achromobacter cycloclastes IAM1013 | P94127 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| anaerobical purification of N2OR under an Ar atmosphere by three different steps of anion exchange chromatography, followed by cytochrome c affinity chromatography and ultrafiltration | Achromobacter cycloclastes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 124 | - |
purified enzyme, pH 8.0, 25°C | Achromobacter cycloclastes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrous oxide + reduced methyl viologen | - |
Achromobacter cycloclastes | nitrogen + H2O + methyl viologen | - |
? | |
| nitrous oxide + reduced methyl viologen | - |
Achromobacter cycloclastes IAM1013 | nitrogen + H2O + methyl viologen | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | perturbations of the protein conformation induced by pH variations, although the principal secondary structure elements are largely unaltered | Achromobacter cycloclastes |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| N2OR | - |
Achromobacter cycloclastes |
| nitrous oxide reductase | - |
Achromobacter cycloclastes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Achromobacter cycloclastes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.1 | 8 | assay at | Achromobacter cycloclastes |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.7 | 10.6 | perturbations of the protein conformation induced by pH variations, although the principal secondary structure elements are largely unaltered | Achromobacter cycloclastes |
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Release 2023.1 (January 2023)
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