Literature summary for 1.7.2.4 extracted from

Wunsch, P.; Koerner, H.; Neese, F.; van Spanning, R.J.; Kroneck, P.M.; Zumft, W.G.
NosX function connects to nitrous oxide (N2O) reduction by affecting the Cu(Z) center of NosZ and its activity in vivo (2005), FEBS Lett., 579, 4605-4609.

Search for more literature for 1.7.2.4

Protein Variants

Protein Variants	Comment	Organism
additional information	strains with a nosXnirX double mutation or nirX single mutation show loss of N2O respiration	Paracoccus denitrificans

Organism

Organism	UniProt	Comment	Textmining
Paracoccus denitrificans	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
HiTrap Q Sepharose anion exchange chromatography	Paracoccus denitrificans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.97	-	wild type enzyme	Paracoccus denitrificans
3.52	-	single mutant nirX	Paracoccus denitrificans
3.63	-	double mutant nosXnirX	Paracoccus denitrificans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N2O + reduced acceptor	-	Paracoccus denitrificans	N2 + H2O + acceptor	-	?

Synonyms

Synonyms	Comment	Organism
N2O reductase	-	Paracoccus denitrificans
N2OR	-	Paracoccus denitrificans
NosZ	-	Paracoccus denitrificans

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Release 2023.1 (January 2023)