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Literature summary for 1.7.2.4 extracted from
- Characterization of nitrous oxide reductase from a methylotrophic denitrifying bacterium, Hyphomicrobium denitrificans A3151 (2003), J. Biochem., 134, 853-858.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| copper | form A and B contain 9.0 and 8.2 Cu atoms per dimer, respectively | Hyphomicrobium denitrificans |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
2 * 65000, SDS-PAGE | Hyphomicrobium denitrificans |
| 105000 | - |
gel filtration | Hyphomicrobium denitrificans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N2O + reduced acceptor | Hyphomicrobium denitrificans | - |
N2 + H2O + acceptor | - |
? | |
| N2O + reduced acceptor | Hyphomicrobium denitrificans A3151 | - |
N2 + H2O + acceptor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hyphomicrobium denitrificans | - |
A3151 | - |
| Hyphomicrobium denitrificans A3151 | - |
A3151 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, DEAE-Sephadex A-50, Sephacryl S-200 | Hyphomicrobium denitrificans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 29 | - |
HdN2OR form A, i.e. reduced form | Hyphomicrobium denitrificans |
| 45 | - |
HdN2OR form A, i.e. oxidized form | Hyphomicrobium denitrificans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N2O + reduced acceptor | - |
Hyphomicrobium denitrificans | N2 + H2O + acceptor | - |
? | |
| N2O + reduced acceptor | - |
Hyphomicrobium denitrificans A3151 | N2 + H2O + acceptor | - |
? | |
| N2O + reduced benzyl viologen | - |
Hyphomicrobium denitrificans | N2 + H2O + benzyl viologen | - |
? | |
| N2O + reduced benzyl viologen | - |
Hyphomicrobium denitrificans A3151 | N2 + H2O + benzyl viologen | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 65000, SDS-PAGE | Hyphomicrobium denitrificans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HdN2OR | - |
Hyphomicrobium denitrificans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | - |
- |
Hyphomicrobium denitrificans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.5 | approx. 50% of maximal activity at pH 7.5, approx. 75% of maximal activity at pH 9.5 | Hyphomicrobium denitrificans |
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