Inhibitors | Comment | Organism | Structure |
---|---|---|---|
O2 | unpurified enzyme is rapid inactivated at air | Pseudomonas stutzeri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu | 8 atoms per mol enzyme | Pseudomonas stutzeri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74000 | - |
2 * 74000, SDS-PAGE | Pseudomonas stutzeri |
118000 | - |
gel filtration | Pseudomonas stutzeri |
130000 | - |
non denaturating PAGE | Pseudomonas stutzeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrous oxide + reduced acceptor | Pseudomonas stutzeri | - |
nitrogen + H2O + acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas stutzeri | - |
ATCC 14405 | - |
Purification (Comment) | Organism |
---|---|
a pink form is isolated when the purification is done aerobically, a purple form when the purification is under anaerobic conditions | Pseudomonas stutzeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrous oxide + reduced acceptor | - |
Pseudomonas stutzeri | nitrogen + H2O + acceptor | - |
? | |
nitrous oxide + reduced acceptor | photochemically-reduced benzyl viologen as electron donor | Pseudomonas stutzeri | nitrogen + H2O + acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 74000, SDS-PAGE | Pseudomonas stutzeri |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
85% inactivation after 20 h | Pseudomonas stutzeri |