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Literature summary for 1.7.2.4 extracted from
- Purification and some characteristics of a cytochrome c-containing nitrous oxide reductase from Wolinella succinogenes (1989), J. Biol. Chem., 264, 1972-1979.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
benzyl viologen | - |
Wolinella succinogenes |  |
| 0.0075 | - |
N2O | - |
Wolinella succinogenes | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1.2 atoms per subunit | Wolinella succinogenes | |
| Cu | 2.9 atoms per subunit | Wolinella succinogenes | |
| Fe | 1 atom per mol | Wolinella succinogenes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 88000 | - |
2 * 88000, SDS-PAGE | Wolinella succinogenes |
| 162000 | - |
gel filtration | Wolinella succinogenes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrous oxide + reduced acceptor | Wolinella succinogenes | - |
nitrogen + H2O + acceptor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Wolinella succinogenes | - |
under anaerobic conditions cytochrome c oxidase activity is observed | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification under anaerobic conditions | Wolinella succinogenes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 160 | - |
- |
Wolinella succinogenes |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, under aerobic conditions, 100 h stable | Wolinella succinogenes |
| 4°C, under aerobic conditions, 5 mM EDTA, 3 h stable | Wolinella succinogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N2O + reduced benzyl viologen | - |
Wolinella succinogenes | N2 + H2O + benzyl viologen | - |
? | |
| nitrous oxide + reduced acceptor | - |
Wolinella succinogenes | nitrogen + H2O + acceptor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 88000, SDS-PAGE | Wolinella succinogenes |
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