Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.7.2.4 extracted from

  • Sato, K.; Okubo, A.; Yamazaki, S.
    Anaerobic purification and characterization of nitrous oxide reductase from Rhodobacter sphaeroides f. sp. denitrificans IL106 (1999), J. Biochem., 125, 864-868.
    View publication on PubMed

General Stability

General Stability Organism
turnover dependent inactivation, promoted by zinc ions at 0.01 mM Cereibacter sphaeroides

Inhibitors

Inhibitors Comment Organism Structure
O2 turnover dependent inactivation Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66180
-
2 * 66180, MALDI-TOF Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrous oxide + reduced acceptor Cereibacter sphaeroides
-
nitrogen + H2O + acceptor
-
?

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
f. sp. denitrificans IL106
-

Purification (Commentary)

Purification (Comment) Organism
purification under anaerobic conditions Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
44
-
aerobic form Cereibacter sphaeroides
78
-
anaerobic form Cereibacter sphaeroides

Storage Stability

Storage Stability Organism
4°C, retained most of its activity after aerobic storage for 2 months without any additives Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrous oxide + reduced acceptor
-
Cereibacter sphaeroides nitrogen + H2O + acceptor
-
?
nitrous oxide + reduced acceptor dithionite-reduced benzyl viologen as electron donor Cereibacter sphaeroides nitrogen + H2O + acceptor
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 66180, MALDI-TOF Cereibacter sphaeroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Cereibacter sphaeroides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
most of activity lost Cereibacter sphaeroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9 with reduced benzyl viologen as electron donor Cereibacter sphaeroides