Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCN | 10-15% inhibition of the inducible and of the constitutive enzyme at 10 mM | Escherichia coli | |
N-ethylmaleimide | both inducible and constitutive enzyme are affected equally at 5 mM | Escherichia coli | |
NaN3 | 5% inhibition of inducible enzyme and 50-60% inhibition of constitutive enzyme at 10 mM | Escherichia coli | |
p-chloromercuribenzoate | 2% inhibition of inducible enzyme and 50-60% inhibition of constitutive enzyme at 5 mM | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | constitutive enzyme | Escherichia coli | 16020 | - |
soluble | inducible enzyme | Escherichia coli | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
a constitutive enzyme and an inducible enzyme with different properties | - |
Oxidation Stability | Organism |
---|---|
aeration for 10 min causes 10% inactivation | Escherichia coli |
Storage Stability | Organism |
---|---|
-80°C, inducible and constitutive enzyme, 10% loss of activity after 15 days | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trimethylamine N-oxide + electron donor | benzyl viologen as electron donor | Escherichia coli | trimethylamine + oxidized electron donor + H2O | - |
? | |
trimethylamine N-oxide + electron donor | NADH as electron donor | Escherichia coli | trimethylamine + oxidized electron donor + H2O | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
20 min, 10% loss of activity | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
for inducible and constitutive enzyme | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Escherichia coli |