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Literature summary for 1.7.2.3 extracted from
- Purification and properties of trimethylamine oxide reductase from Salmonella typhimurium (1983), J. Bacteriol., 155, 1455-1458.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | not inhibitory at 1 mM | Salmonella enterica subsp. enterica serovar Typhimurium |  |
| Cu2+ | 50% inhibition at 0.1 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
| EDTA | not inhibitory at 1 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
| FAD | inhibition, when added to the standard assay mixture containing benzyl viologen as electron acceptor | Salmonella enterica subsp. enterica serovar Typhimurium | |
| FMN | inhibition, when added to the standard assay mixture containing benzyl viologen as electron acceptor | Salmonella enterica subsp. enterica serovar Typhimurium | |
| Hg2+ | 80% inhibition at 1 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
| KCN | not inhibitory at 5 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
| Mn2+ | not inhibitory at 1 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
| NaN3 | not inhibitory at 10 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.89 | - |
Trimethylamine N-oxide | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| 2.2 | - |
Chlorate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 84000 | - |
4 * 84000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
| 332000 | - |
gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
inducible enzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using polyethyleneimine to remove nucleic acids, ammonium sulfate treatment, column chromatography on DEAE-Sephacel and Bio-Gel A-5m, chromatofocusing and chromatography on Sephacryl-S-300 column | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, 10% loss of activity after 15 days | Salmonella enterica subsp. enterica serovar Typhimurium |
| 0°C, 50 mM sodium phosphate buffer, pH 7.3, 30% loss of activity after 17 days | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chlorate + electron donor | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| additional information | when FMN, FAD, cytochrome c, NADPH, NADH or ascorbate are substituted at equimolar concentration for benzyl viologen, none facilitates the oxidation of dithionite by the trimethylamine N-oxide | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| additional information | the enzyme has very slight activity with nitrate, but no activity with nitrite, sulfite or thiosulfate | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| trimethylamine N-oxide + electron donor | benzyl viologen as electron donor | Salmonella enterica subsp. enterica serovar Typhimurium | trimethylamine + oxidized electron donor + H2O | - |
? | |
| trimethylamine N-oxide + electron donor | the activity with 3.4 mM FMN is 17% of that obtained with 0.33 mM benzyl viologen | Salmonella enterica subsp. enterica serovar Typhimurium | trimethylamine + oxidized electron donor + H2O | - |
? | |
| trimethylamine N-oxide + electron donor | NADH as electron donor | Salmonella enterica subsp. enterica serovar Typhimurium | trimethylamine + oxidized electron donor + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 84000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
10 min, pH 7.3, quite stable at temperatures below | Salmonella enterica subsp. enterica serovar Typhimurium |
| 80 | - |
10 min, pH 7.3, rapid loss of activity | Salmonella enterica subsp. enterica serovar Typhimurium |
| 90 | - |
10 min, pH 7.3, complete loss of activity | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.65 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.3 | - |
not stable | Salmonella enterica subsp. enterica serovar Typhimurium |
| 6 | 8.5 | stability peak at pH 7.3 | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
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