Crystallization (Comment) | Organism |
---|---|
purified NrfA, hanging drop vapor diffusion method, five conditions that result in several different crystal packing motifs, e.g. 0.001 ml of protein solution containing 10 mg/ml of NrfA mixed with 0.001 ml of mother liquor containing 100 mM HEPES, pH 7.5, 20% PEG 10000 and incubated in hanging drop trays with a 1 ml well solution at 4°C, 5-14 days, other conditions, overview. X-ray diffraction structure determination and analysis at 1.7-2.5 A resolution | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | EDTA protects NrfA from zinc inhibition, zinc inhibition kinetics in presence or absence of EDTA, overview | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Escherichia coli | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | active site calcium ion | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrite + ferrocytochrome c + H+ | Escherichia coli | the periplasmic cytochrome c nitrite reductase, Nrf, system of Escherichia coli utilizes nitrite as a respiratory electron acceptor by reducing it to ammonium. NO is a proposed intermediate in this six-electron reduction and NrfA can use exogenous NO as a substrate | NH3 + H2O + ferricytochrome c | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0ABK9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrite + ferrocytochrome c + H+ | - |
Escherichia coli | NH3 + H2O + ferricytochrome c | - |
? | |
nitrite + ferrocytochrome c + H+ | the periplasmic cytochrome c nitrite reductase, Nrf, system of Escherichia coli utilizes nitrite as a respiratory electron acceptor by reducing it to ammonium. NO is a proposed intermediate in this six-electron reduction and NrfA can use exogenous NO as a substrate | Escherichia coli | NH3 + H2O + ferricytochrome c | - |
? | |
nitrite + reduced methyl viologen | - |
Escherichia coli | NH3 + H2O + oxidized methyl viologen | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cytochrome c nitrite reductase | - |
Escherichia coli |
NrfA | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
methyl viologen | artificial cofactor, zinc-reduced or dithionite-reduced | Escherichia coli |