Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Nitrososphaera viennensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.287 | - |
nitrite | pH 6.5, 25°C | Nitrososphaera viennensis | |
0.287 | - |
nitrite | at pH 8.0 and 25°C | Nitrososphaera viennensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | 2.9 atoms per subunit | Nitrososphaera viennensis | |
Cu2+ | contains 2.9 atoms per subunit of the recombinant protein | Nitrososphaera viennensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
105000 | - |
gel filtration | Nitrososphaera viennensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrite + ferrocytochrome c + 2 H+ | Nitrososphaera viennensis | - |
nitric oxide + H2O + ferricytochrome c | - |
? | |
nitrite + ferrocytochrome c + 2 H+ | Nitrososphaera viennensis JCM19564 | - |
nitric oxide + H2O + ferricytochrome c | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nitrososphaera viennensis | - |
- |
- |
Nitrososphaera viennensis | A0A060HKX3 | - |
- |
Nitrososphaera viennensis EN76 | A0A060HKX3 | - |
- |
Nitrososphaera viennensis JCM19564 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His60 Ni Superflow resin column chromatography and Superdex 200 gel filtration | Nitrososphaera viennensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme catalyzes the reduction of NO2- to NO, the oxidation of hydroxylamine (NH2OH) to NO, reaction of EC 1.7.2.6, and the production of N2O from NH2OH and NO2 | Nitrososphaera viennensis | ? | - |
- |
|
additional information | enzyme catalyzes the reduction of NO2- to NO, the oxidation of hydroxylamine (NH2OH) to NO, reaction of EC 1.7.2.6, and the production of N2O from NH2OH and NO2 | Nitrososphaera viennensis EN76 | ? | - |
- |
|
nitrite + ferrocytochrome c + 2 H+ | - |
Nitrososphaera viennensis | nitric oxide + H2O + ferricytochrome c | - |
? | |
nitrite + ferrocytochrome c + 2 H+ | - |
Nitrososphaera viennensis JCM19564 | nitric oxide + H2O + ferricytochrome c | - |
? | |
nitrite + reduced benzyl viologen | - |
Nitrososphaera viennensis | NO + oxidized benzyl viologen | - |
? | |
nitrite + reduced benzyl viologen | - |
Nitrososphaera viennensis EN76 | NO + oxidized benzyl viologen | - |
? | |
nitrite + reduced benzyl viologen + 2 H+ | - |
Nitrososphaera viennensis | nitric oxide + H2O + oxidized benzyl viologen | - |
? | |
nitrite + reduced benzyl viologen + 2 H+ | - |
Nitrososphaera viennensis JCM19564 | nitric oxide + H2O + oxidized benzyl viologen | - |
? | |
nitrite + reduced phenazine methosulfate | - |
Nitrososphaera viennensis | NO + oxidized phenazine methosulfate | - |
? | |
nitrite + reduced phenazine methosulfate | - |
Nitrososphaera viennensis EN76 | NO + oxidized phenazine methosulfate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Nitrososphaera viennensis |
? | x * 39700, calculated from amino acid sequence | Nitrososphaera viennensis |
Synonyms | Comment | Organism |
---|---|---|
dissimilatory nitrite reductase | - |
Nitrososphaera viennensis |
NirK | - |
Nitrososphaera viennensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.1 | - |
nitrite | pH 6.5, 25°C | Nitrososphaera viennensis | |
3.1 | - |
nitrite | at pH 8.0 and 25°C | Nitrososphaera viennensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c | - |
Nitrososphaera viennensis |