Literature summary for 1.7.2.1 extracted from

Boulanger, M.J.; Murphy, M.E.
Directing the mode of nitrite binding to a copper-containing nitrite reductase from Alcaligenes faecalis S-6: characterization of an active site isoleucine (2003), Protein Sci., 12, 248-256.

Cloned(Commentary)

Cloned (Comment)	Organism
-	Alcaligenes faecalis

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals are grown at 19°C by hanging drop vapour diffusion using a reservoir of 100 mM sodium acetate, pH 4.7, 6%-10% polyethylene glycol 4000 and 1-5 mM cupric chloride, each drop is made from an equal volume of reservoir and a 15 mg/ml protein stock solution buffered in 10 mM Tris pH 7.0, crystals of mutants diffract to 1.8 A, nitrite-soaked oxidized crystals are obtained by placing crystals in reservoir solution supplemented with 5 mM sodium nitrite	Alcaligenes faecalis

Crystallization (Comment)

Organism

crystals are grown at 19°C by hanging drop vapour diffusion using a reservoir of 100 mM sodium acetate, pH 4.7, 6%-10% polyethylene glycol 4000 and 1-5 mM cupric chloride, each drop is made from an equal volume of reservoir and a 15 mg/ml protein stock solution buffered in 10 mM Tris pH 7.0, crystals of mutants diffract to 1.8 A, nitrite-soaked oxidized crystals are obtained by placing crystals in reservoir solution supplemented with 5 mM sodium nitrite

Alcaligenes faecalis

Protein Variants

Protein Variants	Comment	Organism
I257A	3.7% of wild-type activity	Alcaligenes faecalis
I257G	2.5% of wild-type activity	Alcaligenes faecalis
I257L	26% of wild-type activity	Alcaligenes faecalis
I257M	4% of wild-type activity	Alcaligenes faecalis
I257T	1.4% of wild-type activity	Alcaligenes faecalis
I257V	125% of wild-type activity	Alcaligenes faecalis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
periplasm	-	Alcaligenes faecalis	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
copper	copper containing nitrite reductase	Alcaligenes faecalis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
nitrite + ferrocytochrome c	Alcaligenes faecalis	-	NO + H2O + ferricytochrome c	-	?
nitrite + ferrocytochrome c	Alcaligenes faecalis S-6	-	NO + H2O + ferricytochrome c	-	?

Organism

Organism	UniProt	Comment	Textmining
Alcaligenes faecalis	-	S-6	-
Alcaligenes faecalis S-6	-	S-6	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
417	-	-	Alcaligenes faecalis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
nitrite + ferrocytochrome c	-	Alcaligenes faecalis	NO + H2O + ferricytochrome c	-	?
nitrite + ferrocytochrome c	-	Alcaligenes faecalis S-6	NO + H2O + ferricytochrome c	-	?
nitrite + reduced methyl viologen	-	Alcaligenes faecalis	NO + oxidized methyl viologen + H2O	-	?
nitrite + reduced methyl viologen	-	Alcaligenes faecalis S-6	NO + oxidized methyl viologen + H2O	-	?

Synonyms

Synonyms	Comment	Organism
AfNiR	-	Alcaligenes faecalis