Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | 3.2 copper ions per trimer, most probably 3 type 1 and 3 type 2 copper, respectively | Pseudomonas chlororaphis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37700 | - |
3 * 37700, SDS-PAGE | Pseudomonas chlororaphis |
107000 | - |
gel filtration | Pseudomonas chlororaphis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrite + electron donor | Pseudomonas chlororaphis | - |
NO + oxidized electron donor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas chlororaphis | - |
DSM 50135 | - |
Purification (Comment) | Organism |
---|---|
soluble fraction, DEAE-cellulose, Source 15Q, Sephadex 75 | Pseudomonas chlororaphis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.33 | - |
electron donor azurin | Pseudomonas chlororaphis |
130 | - |
electron donor benzyl viologen | Pseudomonas chlororaphis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrite + electron donor | - |
Pseudomonas chlororaphis | NO + oxidized electron donor | - |
? | |
nitrite + reduced azurin | azurin purified from Pseudomonas chlororaphis | Pseudomonas chlororaphis | NO + oxidized azurin | - |
? | |
nitrite + reduced benzyl viologen | - |
Pseudomonas chlororaphis | NO + oxidized benzyl viologen | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 37700, SDS-PAGE | Pseudomonas chlororaphis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.62 | - |
nitrite | pH 7.0, electron donor azurin | Pseudomonas chlororaphis | |
243 | - |
nitrite | pH 7.0, electron donor benzyl viologen | Pseudomonas chlororaphis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas chlororaphis | isoelectric focusing | 6.2 | 5.4 |