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Literature summary for 1.7.1.B4 extracted from

  • Misal, S.; Lingojwar, D.; Shinde, R.; Gawai, K.
    Purification and characterization of azoreductase from alkaliphilic strain Bacillus badius (2011), Process Biochem., 46, 1264-1269.
No PubMed abstract available
Search for more literature for 1.7.1.B4

Application

Application Comment Organism
degradation strain is able to decolorize azo dyes up to 1000 mg /l in 24 h under aerobic conditions. Cell extracts show NADH dependent oxygen-insensitive azoreductase activity Bacillus badius

Cloned(Commentary)

Cloned (Comment) Organism
-
 Bacillus badius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43000, SDS-PAGE Bacillus badius

Organism

Organism UniProt Comment Textmining
Bacillus badius
-
 strain isolated from pristine alkaline Crater Lake of Lonar, India
-

Purification (Commentary)

Purification (Comment) Organism
partial Bacillus badius

Reaction

Reaction Comment Organism Reaction ID
N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NAD+ = 4-(dimethylamino)azobenzene + 2 NADH + 2 H+ the reaction occurs in the reverse direction to that shown above. Other azo dyes, such as tartrazine, Acid Orange, Orange Yellow and Fast Red E can also be reduced.specific for NAD+, does not use FAD or FMN Bacillus badius
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.371
-
 pH not specified in the publication, temperature not specified in the publication Bacillus badius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acid orange II + NADH + H+
-
 Bacillus badius ? + NAD+
-
 ?
amaranth + NADH + H+ best substrate Bacillus badius ? + NAD+
-
 ?
fast red E + NADH + H+
-
 Bacillus badius ? + NAD+
-
 ?
orange 2R + NADH + H+
-
 Bacillus badius ? + NAD+
-
 ?
orange 37 + NADH + H+
-
 Bacillus badius ? + NAD+
-
 ?
orange M2R + NADH + H+
-
 Bacillus badius ? + NAD+
-
 ?
orange yellow + NADH + H+
-
 Bacillus badius ? + NAD+
-
 ?
red HE3B + NADH + H+
-
 Bacillus badius ? + NAD+
-
 ?
red HE7B + NADH + H+
-
 Bacillus badius ? + NAD+
-
 ?
red M5B + NADH + H+
-
 Bacillus badius ? + NAD+
-
 ?
red M8B + NADH + H+
-
 Bacillus badius ? + NAD+
-
 ?
sunset yellow + NADH + H+
-
 Bacillus badius ? + NAD+
-
 ?
tartrazine + NADH + H+
-
 Bacillus badius ? + NAD+
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Bacillus badius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Bacillus badius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Bacillus badius

Cofactor

Cofactor Comment Organism Structure
additional information no FAD or FMN, no reaction with NADPH Bacillus badius
NADH specific Bacillus badius