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Literature summary for 1.7.1.B3 extracted from

  • Valiauga, B.; Miseviciene, L.; Rich, M.H.; Ackerley, D.F.; Sarlauskas, J.; Cenas, N.
    Mechanism of two-/four-electron reduction of nitroaromatics by oxygen-insensitive nitroreductases the role of a non-enzymatic reduction step (2018), Molecules, 23, 1672 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.7.1.B3

Inhibitors

Inhibitors Comment Organism Structure
ascorbate
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,4,6-trinitrophenyl N-methylnitramine + 4 NADPH + H+ Escherichia coli
-
 ?
-
 ?
2,4,6-trinitrotoluene + 2 NADPH + H+ Escherichia coli
-
 ?
-
 ?
4-nitroacetophenone + NADPH + H+ Escherichia coli
-
 ?
-
 ?
4-nitrobenzoic acid + NADPH + H+ Escherichia coli
-
 ?
-
 ?
nitrobenzene + NADPH + H+ Escherichia coli
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4,6-trinitrophenyl N-methylnitramine + 4 NADPH + H+
-
 Escherichia coli ?
-
 ?
2,4,6-trinitrotoluene + 2 NADPH + H+
-
 Escherichia coli ?
-
 ?
4-nitroacetophenone + NADPH + H+
-
 Escherichia coli ?
-
 ?
4-nitrobenzoic acid + NADPH + H+
-
 Escherichia coli ?
-
 ?
nitrobenzene + NADPH + H+
-
 Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
NfsA
-
 Escherichia coli
NR-A
-
 Escherichia coli
oxygen-insensitive NAD(P)H:nitroreductases
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14.2
-
 nitrobenzene at pH 7.0 and 25°C Escherichia coli
59.4
-
 4-Nitroacetophenone at pH 7.0 and 25°C Escherichia coli
64.3
-
 4-nitrobenzoic acid at pH 7.0 and 25°C Escherichia coli
110
-
 2,4,6-trinitrophenyl N-methylnitramine at pH 7.0 and 25°C Escherichia coli
135.1
-
 2,4,6-trinitrotoluene at pH 7.0 and 25°C Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5
-
 nitrobenzene at pH 7.0 and 25°C Escherichia coli
2.5
-
 4-nitrobenzoic acid at pH 7.0 and 25°C Escherichia coli
150
-
 4-Nitroacetophenone at pH 7.0 and 25°C Escherichia coli
2700
-
 2,4,6-trinitrotoluene at pH 7.0 and 25°C Escherichia coli
7900
-
 2,4,6-trinitrophenyl N-methylnitramine at pH 7.0 and 25°C Escherichia coli