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Literature summary for 1.7.1.7 extracted from

  • Imamura, A.; Okada, T.; Mase, H.; Otani, T.; Kobayashi, T.; Tamura, M.; Kubata, B.K.; Inoue, K.; Rambo, R.P.; Uchiyama, S.; Ishii, K.; Nishimura, S.; Inui, T.
    Allosteric regulation accompanied by oligomeric state changes of Trypanosoma brucei GMP reductase through cystathionine-beta-synthase domain (2020), Nat. Commun., 11, 1837 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.7.1.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Trypanosoma brucei

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant C318A, sitting drop vapor diffusion method, using 0.1 M imidazole-HCl (pH 8.0), 3% (w/v) PEG 3350, and 0.35 M Li2SO4 Trypanosoma brucei

Protein Variants

Protein Variants Comment Organism
C318A inactive Trypanosoma brucei

Inhibitors

Inhibitors Comment Organism Structure
ribavirin 5'-monophosphate
-
 Trypanosoma brucei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
494000
-
 gel filtration Trypanosoma brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GMP + NADPH + H+ Trypanosoma brucei
-
 IMP + NH3 + NADP+
-
 ?
GMP + NADPH + H+ Trypanosoma brucei ILTat
-
 IMP + NH3 + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
-
-
Trypanosoma brucei ILTat
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni Sepharose 6 column chromatography and Sephacryl S-200HR gel filtration Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GMP + NADPH + H+
-
 Trypanosoma brucei IMP + NH3 + NADP+
-
 ?
GMP + NADPH + H+
-
 Trypanosoma brucei ILTat IMP + NH3 + NADP+
-
 ?

Subunits

Subunits Comment Organism
homooctamer
-
 Trypanosoma brucei

Synonyms

Synonyms Comment Organism
GMPR
-
 Trypanosoma brucei
guanosine 5'-monophosphate reductase
-
 Trypanosoma brucei

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Trypanosoma brucei