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Literature summary for 1.7.1.7 extracted from

  • Rosenberg, M.M.; Redfield, A.G.; Roberts, M.F.; Hedstrom, L.
    Dynamic characteristics of guanosine-5'-monophosphate reductase complexes revealed by high-resolution 31P field-cycling NMR relaxometry (2018), Biochemistry, 57, 3146-3154 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.7.1.7

Protein Variants

Protein Variants Comment Organism
D219A the mutant shows reduced affinities for substrate and cofactor compared to the wild type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0032
-
 GMP wild type enzyme, at pH 7.8 and 25°C Homo sapiens
0.015
-
 dGMP wild type enzyme, at pH 7.8 and 25°C Homo sapiens
0.016
-
 NADPH wild type enzyme, with GMP as cosubstrate, at pH 7.8 and 25°C Homo sapiens
0.023
-
 IMP wild type enzyme, at pH 7.8 and 25°C Homo sapiens
0.05
-
 NADP+ wild type enzyme, with IMP and NH3 as cosubstrates, at pH 7.8 and 25°C Homo sapiens
0.101
-
 NADPH wild type enzyme, with GMP as cosubstrate, at pH 7.8 and 25°C Homo sapiens
0.109
-
 NADP+ wild type enzyme, with IMP and NH3 as cosubstrates, at pH 7.8 and 25°C Homo sapiens
0.12
-
 NADPH mutant enzyme D219A, with GMP as cosubstrate, at pH 7.8 and 25°C Homo sapiens
0.14
-
 dIMP wild type enzyme, at pH 7.8 and 25°C Homo sapiens
0.38
-
 GMP mutant enzyme D219A, at pH 7.8 and 25°C Homo sapiens
0.42
-
 NADP+ mutant enzyme D219A, with IMP and NH3 as cosubstrates, at pH 7.8 and 25°C Homo sapiens
1.1
-
 IMP mutant enzyme D219A, at pH 7.8 and 25°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GMP + NADPH + H+ Homo sapiens
-
 IMP + NH3 + NADP+
-
 r
IMP + NH3 + NADP+ Homo sapiens
-
 GMP + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9P2T1
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose resin column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dGMP + NADPH + H+ dGMP is a poor substrate Homo sapiens dIMP + NH3 + NADP+
-
 r
dIMP + NH3 + NADP+ dIMP is a poor substrate Homo sapiens dGMP + NADPH + H+
-
 r
GMP + NADPH + H+
-
 Homo sapiens IMP + NH3 + NADP+
-
 r
IMP + NH3 + NADP+
-
 Homo sapiens GMP + NADPH + H+
-
 r

Synonyms

Synonyms Comment Organism
GMPR
-
 Homo sapiens
guanosine-5'-monophosphate reductase
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Homo sapiens