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Literature summary for 1.7.1.6 extracted from

  • Yang, Y.; Lu, L.; Gao, F.; Zhao, Y.
    Characterization of an efficient catalytic and organic solvent-tolerant azoreductase toward methyl red from Shewanella oneidensis MR-1 (2013), Environ. Sci. Pollut. Res. Int., 20, 3232-3239.
    View publication on PubMed
Search for more literature for 1.7.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Shewanella oneidensis

Inhibitors

Inhibitors Comment Organism Structure
Zn2+ 5 mM, 30% residual activity Shewanella oneidensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0028
-
 Methyl red cosubstrate NADPH, pH 6.5, 25°C Shewanella oneidensis
0.0092
-
 Methyl red cosubstrate NADH, pH 6.5, 25°C Shewanella oneidensis
0.48
-
 NADH pH 6.5, 25°C Shewanella oneidensis
0.941
-
 NADPH pH 6.5, 25°C Shewanella oneidensis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activation, optimum concentration 25 mM giving 126% of initial activity Shewanella oneidensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 x * 27000, SDS-PAGE Shewanella oneidensis

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone 30%, no significant loss of activity Shewanella oneidensis
dimethyl sulfoxide 30%, no significant loss of activity Shewanella oneidensis
Ethanol 20%, no significant loss of activity Shewanella oneidensis
isopropanol 20%, no significant loss of activity Shewanella oneidensis
Methanol 30%, no significant loss of activity Shewanella oneidensis
propanol 10%, no significant loss of activity Shewanella oneidensis

Organism

Organism UniProt Comment Textmining
Shewanella oneidensis Q8E990
-
-
Shewanella oneidensis MR-1 / ATCC 700550 Q8E990
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
156.1
-
 pH 6.5, 25°C Shewanella oneidensis
184.6
-
 presence of 25 microM FMN, pH 6.5, 25°C Shewanella oneidensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl red + NADH + H+
-
 Shewanella oneidensis ? + NAD+
-
 ?
methyl red + NADH + H+
-
 Shewanella oneidensis MR-1 / ATCC 700550 ? + NAD+
-
 ?
methyl red + NADPH + H+
-
 Shewanella oneidensis ? + NADP+
-
 ?
methyl red + NADPH + H+
-
 Shewanella oneidensis MR-1 / ATCC 700550 ? + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 27000, SDS-PAGE Shewanella oneidensis

Synonyms

Synonyms Comment Organism
AzoR
-
 Shewanella oneidensis
SO 4396
-
 Shewanella oneidensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Shewanella oneidensis

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.5 more than 50% of maximum activity Shewanella oneidensis

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Shewanella oneidensis
NADH preferred cofactor Shewanella oneidensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
30
-
 NADPH pH 6.5, 25°C Shewanella oneidensis
210
-
 NADH pH 6.5, 25°C Shewanella oneidensis
6760
-
 Methyl red cosubstrate NADPH, pH 6.5, 25°C Shewanella oneidensis
8970
-
 Methyl red cosubstrate NADH, pH 6.5, 25°C Shewanella oneidensis