Cloned (Comment) | Organism |
---|---|
into the vector pGEX 4T1 | Cereibacter sphaeroides |
Protein Variants | Comment | Organism |
---|---|---|
H75N | mutation decreases the binding of methyl red and nitrofurazone and has no effect on the bining of NADPH | Cereibacter sphaeroides |
K109A | K109 might only be involved in the binding of the 2'-phosphate group of NADPH and have no effect on the binding of NADH | Cereibacter sphaeroides |
K109H | K109 might only be involved in the binding of the 2'-phosphate group of NADPH and have no effect on the binding of NADH | Cereibacter sphaeroides |
Y74W | mutation decreases the binding of methyl red and nitrofurazone and has no effect on the bining of NADPH | Cereibacter sphaeroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
Methyl red | NADH-dependent, mutant K109H | Cereibacter sphaeroides | |
0.05 | - |
NADPH | NADPH-dependent, mutant Y74W | Cereibacter sphaeroides | |
0.06 | - |
Methyl red | NADH-dependent, wild-type enzyme | Cereibacter sphaeroides | |
0.06 | - |
NADPH | NADPH-dependent, wild-type enzyme | Cereibacter sphaeroides | |
0.09 | - |
NADPH | wild-type enzyme | Cereibacter sphaeroides | |
0.1 | - |
nitrofurazone | NADPH-dependent, mutant K109A | Cereibacter sphaeroides | |
0.11 | - |
NADPH | mutant Y74W | Cereibacter sphaeroides | |
0.12 | - |
NADPH | mutant H75N | Cereibacter sphaeroides | |
0.12 | - |
nitrofurazone | NADPH-dependent, mutant K109H | Cereibacter sphaeroides | |
0.13 | - |
nitrofurazone | NADPH-dependent, wild-type enzyme | Cereibacter sphaeroides | |
0.15 | - |
Methyl red | NADH-dependent, mutant K109A | Cereibacter sphaeroides | |
0.2 | - |
NADPH | NADPH-dependent, mutant K109H | Cereibacter sphaeroides | |
0.27 | - |
Methyl red | wild-type enzyme | Cereibacter sphaeroides | |
0.27 | - |
Methyl red | mutant K109H | Cereibacter sphaeroides | |
0.31 | - |
NADPH | mutant K109A | Cereibacter sphaeroides | |
0.34 | - |
NADH | mutant K109H | Cereibacter sphaeroides | |
0.36 | - |
NADH | mutant K109A | Cereibacter sphaeroides | |
0.37 | - |
NADPH | NADPH-dependent, mutant K109A | Cereibacter sphaeroides | |
0.38 | - |
NADH | wild-type enzyme | Cereibacter sphaeroides | |
0.65 | - |
nitrofurazone | NADPH-dependent, mutant Y74W | Cereibacter sphaeroides | |
0.66 | - |
Methyl red | mutant K109A | Cereibacter sphaeroides | |
0.68 | - |
NADPH | mutant K109H | Cereibacter sphaeroides | |
0.8 | - |
Methyl red | mutant H75N | Cereibacter sphaeroides | |
1.21 | - |
Methyl red | mutant Y74W | Cereibacter sphaeroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | Q8GKS3 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl red + NAD(P)H + H+ | - |
Cereibacter sphaeroides | ? | - |
? | |
nitrofurazone + NAD(P)H + H+ | - |
Cereibacter sphaeroides | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
azoreductase | - |
Cereibacter sphaeroides |
AZR | - |
Cereibacter sphaeroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
activity assay | Cereibacter sphaeroides |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | - |
Cereibacter sphaeroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
mutants K109H and H75N | Cereibacter sphaeroides |
7 | - |
activity assay | Cereibacter sphaeroides |
9 | - |
mutants K109A and Y74W | Cereibacter sphaeroides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Cereibacter sphaeroides | |
NADH | - |
Cereibacter sphaeroides | |
NADPH | - |
Cereibacter sphaeroides |