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Literature summary for 1.7.1.6 extracted from

  • Liu, G.; Zhou, J.; Wang, J.; Yan, B.; Li, J.; Lu, H.; Qu, Y.; Jin, R.
    Site-directed mutagenesis of substrate binding sites of azoreductase from Rhodobacter sphaeroides (2008), Biotechnol. Lett., 30, 869-875.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pGEX 4T1 Cereibacter sphaeroides

Protein Variants

Protein Variants Comment Organism
H75N mutation decreases the binding of methyl red and nitrofurazone and has no effect on the bining of NADPH Cereibacter sphaeroides
K109A K109 might only be involved in the binding of the 2'-phosphate group of NADPH and have no effect on the binding of NADH Cereibacter sphaeroides
K109H K109 might only be involved in the binding of the 2'-phosphate group of NADPH and have no effect on the binding of NADH Cereibacter sphaeroides
Y74W mutation decreases the binding of methyl red and nitrofurazone and has no effect on the bining of NADPH Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
Methyl red NADH-dependent, mutant K109H Cereibacter sphaeroides
0.05
-
NADPH NADPH-dependent, mutant Y74W Cereibacter sphaeroides
0.06
-
Methyl red NADH-dependent, wild-type enzyme Cereibacter sphaeroides
0.06
-
NADPH NADPH-dependent, wild-type enzyme Cereibacter sphaeroides
0.09
-
NADPH wild-type enzyme Cereibacter sphaeroides
0.1
-
nitrofurazone NADPH-dependent, mutant K109A Cereibacter sphaeroides
0.11
-
NADPH mutant Y74W Cereibacter sphaeroides
0.12
-
NADPH mutant H75N Cereibacter sphaeroides
0.12
-
nitrofurazone NADPH-dependent, mutant K109H Cereibacter sphaeroides
0.13
-
nitrofurazone NADPH-dependent, wild-type enzyme Cereibacter sphaeroides
0.15
-
Methyl red NADH-dependent, mutant K109A Cereibacter sphaeroides
0.2
-
NADPH NADPH-dependent, mutant K109H Cereibacter sphaeroides
0.27
-
Methyl red wild-type enzyme Cereibacter sphaeroides
0.27
-
Methyl red mutant K109H Cereibacter sphaeroides
0.31
-
NADPH mutant K109A Cereibacter sphaeroides
0.34
-
NADH mutant K109H Cereibacter sphaeroides
0.36
-
NADH mutant K109A Cereibacter sphaeroides
0.37
-
NADPH NADPH-dependent, mutant K109A Cereibacter sphaeroides
0.38
-
NADH wild-type enzyme Cereibacter sphaeroides
0.65
-
nitrofurazone NADPH-dependent, mutant Y74W Cereibacter sphaeroides
0.66
-
Methyl red mutant K109A Cereibacter sphaeroides
0.68
-
NADPH mutant K109H Cereibacter sphaeroides
0.8
-
Methyl red mutant H75N Cereibacter sphaeroides
1.21
-
Methyl red mutant Y74W Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides Q8GKS3
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl red + NAD(P)H + H+
-
Cereibacter sphaeroides ?
-
?
nitrofurazone + NAD(P)H + H+
-
Cereibacter sphaeroides ?
-
?

Synonyms

Synonyms Comment Organism
azoreductase
-
Cereibacter sphaeroides
AZR
-
Cereibacter sphaeroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
activity assay Cereibacter sphaeroides

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50
-
Cereibacter sphaeroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
mutants K109H and H75N Cereibacter sphaeroides
7
-
activity assay Cereibacter sphaeroides
9
-
mutants K109A and Y74W Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
FMN
-
Cereibacter sphaeroides
NADH
-
Cereibacter sphaeroides
NADPH
-
Cereibacter sphaeroides