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Literature summary for 1.7.1.6 extracted from

  • Suzuki, Y.; Yoda, T.; Ruhul, A.; Sugiura, W.
    Molecular cloning and characterization of the gene coding for azoreductase from Bacillus sp. OY1-2 isolated from soil (2001), J. Biol. Chem., 276, 9059-9065.
    View publication on PubMed
Search for more literature for 1.7.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 x * 20000, recombinant and native enzyme, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
 sp. OY1-2 isolated from soil near a waste-water plant
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.6
-
 recombinant enzyme at 50°C with roccelin Bacillus sp. (in: Bacteria)
11.7
-
 native enzyme at 70°C with roccelin Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Rocceline + NADPH
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
Solar Orange + NADPH
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
Sumifix Black B + NADPH
-
 Bacillus sp. (in: Bacteria) ?
-
 ?

Subunits

Subunits Comment Organism
? x * 20000, recombinant and native enzyme, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 recombinant enzyme Bacillus sp. (in: Bacteria)
70
-
 native enzyme Bacillus sp. (in: Bacteria)