Activating Compound | Comment | Organism | Structure |
---|---|---|---|
FMN | increases activity | Bacillus cereus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, 92% inhibition | Bacillus cereus | |
Cu2+ | 1 mM, 88% inhibition | Bacillus cereus | |
Fe2+ | 1 mM, 90% inhibition | Bacillus cereus | |
Fe3+ | 1 mM, 85% inhibition | Bacillus cereus | |
Hg2+ | 1 mM, 75% inhibition | Bacillus cereus | |
additional information | not inhibited by N-ethylmaleimide, p-chloromercuribenzoate and monoiodoacetic acid | Bacillus cereus | |
SDS | 1 mM, 50% inhibition | Bacillus cereus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Bacillus cereus | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | slight activation | Bacillus cereus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
T-105 | - |
Bacillus cereus T-105 | - |
T-105 | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus cereus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.566 | - |
- |
Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-(dimethylamino)azobenzene + 2 NADPH | - |
Bacillus cereus | N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+ | - |
? | |
4-(dimethylamino)azobenzene + 2 NADPH | - |
Bacillus cereus T-105 | N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+ | - |
? | |
new coccine + NADPH | - |
Bacillus cereus | ? | - |
? | |
new coccine + NADPH | - |
Bacillus cereus T-105 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Bacillus cereus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 70 | at 5°C and 70°C: about 55% of maximal activity | Bacillus cereus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
10 min, stable up to | Bacillus cereus |
80 | - |
10 min, 45% loss of activity | Bacillus cereus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Bacillus cereus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | pH 4.0: about 10% activity, pH 10.0: about 20% activity | Bacillus cereus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | 5°C, 7 days, stable | Bacillus cereus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | increases activity | Bacillus cereus | |
flavin | prosthetic group seems to be flavin | Bacillus cereus | |
additional information | no activity with NADH | Bacillus cereus | |
NADPH | specific for | Bacillus cereus | |
riboflavin | no activation | Bacillus cereus |