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Literature summary for 1.7.1.4 extracted from
- A nitrite reductase from Neurospora crassa (1960), Biochim. Biophys. Acta, 37, 468-476.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| FMN | addition increases activity | Neurospora crassa |  |
| pyridoxine | stimulates activity in crude extracts from pyridoxine-deficient felts, no activation of purified enzyme | Neurospora crassa | |
General Stability
| General Stability | Organism |
|---|---|
| dialysis against water or a variety of buffers deactivates | Neurospora crassa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Neurospora crassa | |
| 2,2'-bipyridine | - |
Neurospora crassa | |
| 2-heptyl-4-hydroxyquinoline N-oxide | - |
Neurospora crassa | |
| 8-hydroxyquinoline | - |
Neurospora crassa | |
| Atabrine | - |
Neurospora crassa | |
| CN- | - |
Neurospora crassa | |
| Diquinolyl | - |
Neurospora crassa | |
| EDTA | - |
Neurospora crassa | |
| Hydrazine sulfate | complete inhibition | Neurospora crassa | |
| Iodosobenzoate | - |
Neurospora crassa | |
| naphthoquinone | - |
Neurospora crassa | |
| p-chloromercuribenzoate | completely reversed by glutathione | Neurospora crassa | |
| salicylic acid | - |
Neurospora crassa | |
| Sodium diethyldithiocarbamate | - |
Neurospora crassa | |
| Thiourea | - |
Neurospora crassa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
FAD | - |
Neurospora crassa | |
| 0.05 | - |
FMN | - |
Neurospora crassa | |
| 0.1 | - |
NO2- | - |
Neurospora crassa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | depressed activity in Cu-deficient felt extracts, stimulates activity in extracts from Cu-deficient felts, depresses activity in extracts from control felts | Neurospora crassa | |
| Fe3+ | stimulates activity in Fe-deficient felt extracts | Neurospora crassa | |
| Magnesium | depressed activity in Mg-deficient felt extracts | Neurospora crassa | |
| Zinc | depressed activity in Zn-deficient felt extracts | Neurospora crassa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Neurospora crassa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0523 | - |
- |
Neurospora crassa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -17°C, overnight, 50% loss of activity | Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NAD(P)H + nitrite + H+ | NADH is preferred | Neurospora crassa | NAD(P)+ + NH3 + H2O | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Neurospora crassa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 11 | pH 4.0: 40% activity, pH 11.0: 70% activity | Neurospora crassa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | increases activity | Neurospora crassa | |
| NADH | - |
Neurospora crassa | |
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Release 2023.1 (January 2023)
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