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Literature summary for 1.7.1.4 extracted from

  • Coleman, K.J.; Cornish-Bowden, A.; Cole, J.A.
    Purification and properties of nitrite reductase from Escherichia coli K12 (1978), Biochem. J., 175, 483-493.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Escherichia coli
2,2'-bipyridine
-
Escherichia coli
arsenite 10 mM, 24% inhibition Escherichia coli
CN-
-
Escherichia coli
NaN3
-
Escherichia coli
p-chloromercuribenzoate 0.02 mM, complete inactivation, partially prevented by 10 mM cysteine Escherichia coli
SO32- 1 mM, 22% inhibition Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cell associated
-
Escherichia coli
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
88000
-
2 * 88000, SDS-PAGE Escherichia coli
190000
-
glycerol density gradient centrifugation Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NAD(P)H + nitrite + H+ Escherichia coli
-
NAD(P)+ + NH3 + H2O
-
?
NAD(P)H + nitrite + H+ Escherichia coli OR75Ch15
-
NAD(P)+ + NH3 + H2O
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli OR75Ch15
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-cellulose, DEAE-Sephadex Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.7
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD(P)H + NH2OH
-
Escherichia coli NAD(P)+ + NH3 + H2O
-
?
NAD(P)H + NH2OH
-
Escherichia coli OR75Ch15 NAD(P)+ + NH3 + H2O
-
?
NAD(P)H + nitrite + H+
-
Escherichia coli NAD(P)+ + NH3 + H2O
-
?
NAD(P)H + nitrite + H+
-
Escherichia coli OR75Ch15 NAD(P)+ + NH3 + H2O
-
?

Subunits

Subunits Comment Organism
dimer 2 * 88000, SDS-PAGE Escherichia coli

Cofactor

Cofactor Comment Organism Structure
flavin 0.4 mol of flavin per mol of enzyme Escherichia coli
additional information heme is not detectable Escherichia coli
NADH
-
Escherichia coli