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Literature summary for 1.7.1.3 extracted from

  • Gholami-Shabani, M.; Akbarzadeh, A.; Norouzian, D.; Amini, A.; Gholami-Shabani, Z.; Imani, A.; Chiani, M.; Riazi, G.; Shams-Ghahfarokhi, M.; Razzaghi-Abyaneh, M.
    Antimicrobial activity and physical characterization of silver nanoparticles green synthesized using nitrate reductase from Fusarium oxysporum (2014), Appl. Biochem. Biotechnol., 172, 4084-4098 .
    View publication on PubMed

Application

Application Comment Organism
medicine the purified native enzyme is successfully used for synthesis of silver nanoparticles in an NADPH-dependent manner using gelatin as a capping agent, analysis by X-ray diffraction, dynamic light scattering spectroscopy, and transmission and scanning electron microscopy, overview. The stable nonaggregating nanoparticles are spherical in shape with an average size of 50 nm and a zeta potential of -34.3. The synthesized nanoparticles show a strong growth inhibitory antimicrobial activity against all tested human pathogenic fungi and bacteria, overview Fusarium oxysporum
synthesis the purified native enzyme is successfully used for synthesis of silver nanoparticles in an NADPH-dependent manner using gelatin as a capping agent, analysis by X-ray diffraction, dynamic light scattering spectroscopy, and transmission and scanning electron microscopy, overview. The stable nonaggregating nanoparticles are spherical in shape with an average size of 50 nm and a zeta potential of -34.3. The synthesized nanoparticles show a strong growth inhibitory antimicrobial activity against all tested human pathogenic fungi and bacteria, overview Fusarium oxysporum

Protein Variants

Protein Variants Comment Organism
additional information the purified native enzyme is successfully used for synthesis of silver nanoparticles in an NADPH-dependent manner using gelatin as a capping agent, analysis by X-ray diffraction, dynamic light scattering spectroscopy, and transmission and scanning electron microscopy, overview. 0.2 M Phosphate buffer, pH 7.2, 1 mM silver nitrate as the enzyme substrate, 0.1 mg gelatin as a capping agent, 1 mM 4-hydroxyquinoline as an electron carrier, 1 mM NADPH as enzyme cofactor, and 0.1 mg of purified fungal nitrate reductase are incubated at 25°C for 5 h. The stable nonaggregating nanoparticles are spherical in shape with an average size of 50 nm and a zeta potential of -34.3. The synthesized nanoparticles show a strong growth inhibitory antimicrobial activity against all tested human pathogenic fungi and bacteria, overview Fusarium oxysporum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
214000
-
gel filtration Fusarium oxysporum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrate + NADPH + H+ Fusarium oxysporum
-
nitrite + NADP+ + H2O
-
?
nitrate + NADPH + H+ Fusarium oxysporum IRAN 31C
-
nitrite + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Fusarium oxysporum
-
-
-
Fusarium oxysporum IRAN 31C
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 70fold by ultrafiltration, anion exchange chromatography, and gel filtration Fusarium oxysporum

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Fusarium oxysporum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
177.1
-
purified native enzyme, substrate nitrate, pH 7.2, 37°C Fusarium oxysporum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrate + NADPH + H+
-
Fusarium oxysporum nitrite + NADP+ + H2O
-
?
nitrate + NADPH + H+
-
Fusarium oxysporum IRAN 31C nitrite + NADP+ + H2O
-
?
nitrate + reduced methyl viologen
-
Fusarium oxysporum nitrite + oxidized methyl viologen
-
?
nitrate + reduced methyl viologen
-
Fusarium oxysporum IRAN 31C nitrite + oxidized methyl viologen
-
?

Subunits

Subunits Comment Organism
heterotrimer 1 * 125000 + 1 * 60000 + 1 * 25000, SDS-PAGE Fusarium oxysporum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Fusarium oxysporum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 60 purified native nitrate reductase, pH 7.2, maintaining 50% of its original activity Fusarium oxysporum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Fusarium oxysporum

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 purified native nitrate reductase, maintaining 50% of its original activity Fusarium oxysporum

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Fusarium oxysporum