Application | Comment | Organism |
---|---|---|
medicine | the purified native enzyme is successfully used for synthesis of silver nanoparticles in an NADPH-dependent manner using gelatin as a capping agent, analysis by X-ray diffraction, dynamic light scattering spectroscopy, and transmission and scanning electron microscopy, overview. The stable nonaggregating nanoparticles are spherical in shape with an average size of 50 nm and a zeta potential of -34.3. The synthesized nanoparticles show a strong growth inhibitory antimicrobial activity against all tested human pathogenic fungi and bacteria, overview | Fusarium oxysporum |
synthesis | the purified native enzyme is successfully used for synthesis of silver nanoparticles in an NADPH-dependent manner using gelatin as a capping agent, analysis by X-ray diffraction, dynamic light scattering spectroscopy, and transmission and scanning electron microscopy, overview. The stable nonaggregating nanoparticles are spherical in shape with an average size of 50 nm and a zeta potential of -34.3. The synthesized nanoparticles show a strong growth inhibitory antimicrobial activity against all tested human pathogenic fungi and bacteria, overview | Fusarium oxysporum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the purified native enzyme is successfully used for synthesis of silver nanoparticles in an NADPH-dependent manner using gelatin as a capping agent, analysis by X-ray diffraction, dynamic light scattering spectroscopy, and transmission and scanning electron microscopy, overview. 0.2 M Phosphate buffer, pH 7.2, 1 mM silver nitrate as the enzyme substrate, 0.1 mg gelatin as a capping agent, 1 mM 4-hydroxyquinoline as an electron carrier, 1 mM NADPH as enzyme cofactor, and 0.1 mg of purified fungal nitrate reductase are incubated at 25°C for 5 h. The stable nonaggregating nanoparticles are spherical in shape with an average size of 50 nm and a zeta potential of -34.3. The synthesized nanoparticles show a strong growth inhibitory antimicrobial activity against all tested human pathogenic fungi and bacteria, overview | Fusarium oxysporum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
214000 | - |
gel filtration | Fusarium oxysporum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + NADPH + H+ | Fusarium oxysporum | - |
nitrite + NADP+ + H2O | - |
? | |
nitrate + NADPH + H+ | Fusarium oxysporum IRAN 31C | - |
nitrite + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium oxysporum | - |
- |
- |
Fusarium oxysporum IRAN 31C | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 70fold by ultrafiltration, anion exchange chromatography, and gel filtration | Fusarium oxysporum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Fusarium oxysporum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
177.1 | - |
purified native enzyme, substrate nitrate, pH 7.2, 37°C | Fusarium oxysporum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + NADPH + H+ | - |
Fusarium oxysporum | nitrite + NADP+ + H2O | - |
? | |
nitrate + NADPH + H+ | - |
Fusarium oxysporum IRAN 31C | nitrite + NADP+ + H2O | - |
? | |
nitrate + reduced methyl viologen | - |
Fusarium oxysporum | nitrite + oxidized methyl viologen | - |
? | |
nitrate + reduced methyl viologen | - |
Fusarium oxysporum IRAN 31C | nitrite + oxidized methyl viologen | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotrimer | 1 * 125000 + 1 * 60000 + 1 * 25000, SDS-PAGE | Fusarium oxysporum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Fusarium oxysporum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | purified native nitrate reductase, pH 7.2, maintaining 50% of its original activity | Fusarium oxysporum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Fusarium oxysporum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | purified native nitrate reductase, maintaining 50% of its original activity | Fusarium oxysporum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Fusarium oxysporum |